A multicolour flow cytometric assay for c-MYC protein in B-cell lymphoma
| Autor: | Samir Turakhia, Shashirekha Shetty, Howard J. Meyerson, Khaled Alayed, Amad Awadallah, Karen Schweitzer |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Lymphoma B-Cell Cell CD19 Immunophenotyping Pathology and Forensic Medicine Flow cytometry Proto-Oncogene Proteins c-myc 03 medical and health sciences 0302 clinical medicine medicine Humans Child B-cell lymphoma Aged Aged 80 and over medicine.diagnostic_test biology Chemistry General Medicine Gene rearrangement Middle Aged Flow Cytometry medicine.disease Molecular biology Staining Lymphoma 030104 developmental biology medicine.anatomical_structure Child Preschool 030220 oncology & carcinogenesis biology.protein Immunohistochemistry Female |
| Zdroj: | Journal of Clinical Pathology. 71:906-915 |
| ISSN: | 1472-4146 0021-9746 |
| Popis: | AimDevelop an objective assay to detect c-MYC protein expression using multiparametric flow cytometry (FCM) as an alternative to immunohistochemistry (IHC).Methods57 patient samples and 11 cell line samples were evaluated. Cell suspensions were obtained and c-MYC staining was performed in combination with CD45 and CD19 and, in some samples, CD10. The percentage of c-MYC+ cells by FCM was correlated with the percentage determined by IHC. The relationship between c-MYC protein expression and the presence of ac-MYCgene rearrangement in aggressive and high-grade lymphomas was also assessed.Resultsc-MYC expression by FCM and IHC demonstrated a high degree of correlation in a training set of 33 patient cases, r=0.92, 11 cell line samples, r=0.81 and in a validation set of 24 aggressive and high-grade B-cell lymphomas, r=0.85.c-MYCgene was rearranged by fluorescence in situ hybridisation in 6/9 samples with high c-MYC expression (>40%) by FCM and 6/14 by IHC.ConclusionsWe have developed a reliable multicolour FCM assay to detect c-MYC expression suitable for clinical laboratories that should be helpful to accurately quantify c-MYC expression in B-cell lymphomas. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|