Spin echo nuclear magnetic resonance studies on intact erythrocytes: changes in cellular metabolism as a consequence of carbimazole therapy
| Autor: | John Reglinski, William Ewen Smith, Rhoda Wilson, L. M. Buchanan, James H. McKillop, J.A. Thomson |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
Male medicine.medical_specialty Erythrocytes Magnetic Resonance Spectroscopy Carbimazole Endocrinology Diabetes and Metabolism medicine.medical_treatment Deiodinase Thyrotropin Trab chemistry.chemical_compound Endocrinology Nuclear magnetic resonance Internal medicine medicine Humans Autoantibodies Red Cell biology Chemistry Antithyroid agent Thyroid Glutathione Middle Aged Graves Disease Thyroxine medicine.anatomical_structure biology.protein Triiodothyronine Female Oxidation-Reduction medicine.drug Hormone Immunoglobulins Thyroid-Stimulating |
| Zdroj: | Clinical endocrinology. 37(4) |
| ISSN: | 0300-0664 |
| Popis: | OBJECTIVE Because the exact mechanism of action of carbimazole is uncertain, nuclear magnetic resonance (NMR) spectroscopy was used to investigate cellular changes in erythrocytes from Graves' patients following a course of carbimazole therapy. DESIGN NMR spectroscopy was carried out using intact erythrocytes obtained from Graves' patients prior to and at 2 and 12 months after carbimazole treatment. The data were correlated with thyroid hormone and antibody levels. PATIENTS Twenty patients (four males; 16 females) with newly diagnosed and previously untreated Graves' disease were enrolled into the study. Assessments were made prior to the commencement of therapy and after 2 and 12 months on treatment. Of the 20 patients assessed at 0 and 2 months only 12 completed the study. MEASUREMENTS The oxidation-reduction balance of erythrocyte glutathione was measured directly using 1H spin echo NMR spectroscopy of intact cells. Thyroid hormone and antibody levels were measured using reported methods. RESULTS At 2 and 12 months a significant (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text