Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning
| Autor: | Azita Leavitt, Yoav Peleg, Hila Sberro, Ruth Kiro, Udi Qimron, Eugene Koh, Rotem Sorek |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Phage display
Time Factors T7 phage Bacterial Toxins Virulence Bacterial genome size Biology medicine.disease_cause Article Microbiology 03 medical and health sciences Bacteriophage T7 medicine Escherichia coli Cloning Molecular Molecular Biology Gene 030304 developmental biology 0303 health sciences 030306 microbiology Shotgun sequencing Escherichia coli Proteins Reproducibility of Results Cell Biology Sequence Analysis DNA biology.organism_classification Multigene Family Host-Pathogen Interactions Antitoxins Antitoxin Genome Bacterial |
| Zdroj: | Sberro, Hila; Leavitt, Azita; Kiro, Ruth; Koh, Eugene; Peleg, Yoav; Qimron, Udi; et al.(2013). Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning. Molecular Cell, 50(1). Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/5qg388pt |
| Popis: | Summary Toxin-antitoxin (TA) modules, composed of a toxic protein and a counteracting antitoxin, play important roles in bacterial physiology. We examined the experimental insertion of 1.5 million genes from 388 microbial genomes into an Escherichia coli host using more than 8.5 million random clones. This revealed hundreds of genes (toxins) that could only be cloned when the neighboring gene (antitoxin) was present on the same clone. Clustering of these genes revealed TA families widespread in bacterial genomes, some of which deviate from the classical characteristics previously described for such modules. Introduction of these genes into E. coli validated that the toxin toxicity is mitigated by the antitoxin. Infection experiments with T7 phage showed that two of the new modules can provide resistance against phage. Moreover, our experiments revealed an "antidefense" protein in phage T7 that neutralizes phage resistance. Our results expose active fronts in the arms race between bacteria and phage. |
| Databáze: | OpenAIRE |
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