Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production
| Autor: | Zhanju Liu, Heather L. Evans-Marin, Yingzi Cong, Mingming Sun, Han Liu, Ye Zhao, Wei Wu, Suxia Yao, Hou Pu Liu, L. Wayne Duck, Charles O. Elson, Feidi Chen, Anthony T. Cao, Qing Zhao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Immunology Cell Gram-Positive Bacteria Lymphocyte Activation Article Microbiology Mice 03 medical and health sciences Th2 Cells 0302 clinical medicine Antigen Transforming Growth Factor beta medicine Animals Immunology and Allergy Symbiosis Cells Cultured Interleukin 4 Mucous Membrane biology Lachnospiraceae Cell Differentiation Dendritic Cells Dendritic cell Transforming growth factor beta Th1 Cells biology.organism_classification 030104 developmental biology medicine.anatomical_structure biology.protein Th17 Cells Interleukin-4 Bacteria 030215 immunology |
| Zdroj: | European Journal of Immunology. 46:1162-1167 |
| ISSN: | 0014-2980 |
| Popis: | It has been shown that while commensal bacteria promote Th1, Th17 and Treg cells in lamina propria (LP) in steady-state conditions, they suppress mucosal Th2 cells. However, it is still unclear whether there are specific commensal organisms down-regulating Th2 responses, and the mechanism involved. Here we demonstrate that commensal A4 bacteria, a member of the Lachnospiraceae family, which produce an immunodominant microbiota CBir1 antigen, inhibits LP Th2-cell development. When transferred into the intestines of RAG(-/-) mice, CBir1-specific T cells developed predominately towards Th1 cells and Th17 cells, but to a lesser extent into Th2 cells. The addition of A4 bacterial lysates to CD4(+) T-cell cultures inhibited production of IL-4. A4 bacteria stimulated dendritic cell production of TGF-β, and blockade of TGF-β abrogated A4 bacteria inhibition of Th2-cell development in vitro and in vivo. Collectively, our data show that A4 bacteria inhibit Th2-cell differentiation by inducing dendritic cell production of TGF-β. |
| Databáze: | OpenAIRE |
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