Levamisole effects on major histocompatibility complex and adhesion molecule expression and on myeloid cell adhesion to human colon tumor cell lines
| Autor: | M C Fisher, E S Kimball |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Integrins Umbilical Veins Lymphocyte Receptors Lymphocyte Homing Genes MHC Class I Vascular Cell Adhesion Molecule-1 Biology Integrin alpha4beta1 Immunoenzyme Techniques HT29 Cells Antigen Adjuvants Immunologic MHC class I medicine Cell Adhesion Leukocytes Tumor Cells Cultured Cytotoxic T cell Humans Lymphocyte function-associated antigen 1 Cell adhesion Melanoma Analysis of Variance Cell adhesion molecule Intercellular Adhesion Molecule-1 Lymphocyte Function-Associated Antigen-1 Up-Regulation Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Levamisole Immunology Colonic Neoplasms Cancer research biology.protein Endothelium Vascular Cell Adhesion Molecules |
| Zdroj: | Journal of the National Cancer Institute. 88(2) |
| ISSN: | 0027-8874 |
| Popis: | Background: The drug levamisole has been successfully used in combination with fluorouracil to increase the disease-free interval and survival of patients who have undergone surgical resection of Dukes' stage C colon cancer. Levamisole is thought to affect the host immune response. Several recent studies have examined its effect on the expression of major histocompatibility complex (MHC) class I molecules, but the results have been inconsistent. An equally important requirement for a host cellular immune response is the adhesion of leukocytes to tumor cells. The latter may be required for cell-mediated antitumor cytotoxic responses. Purpose: We evaluated the ability of levamisole to affect the expression of MHC class I molecules and cell-adhesion molecules and determined whether levamisole could affect leukocyte adhesion to tumor cells that had been treated with the drug. Methods: A panel of four human colon tumor cell lines (HT-29, SW-620, HCT-15, and LoVo), A-375 human melanoma cells, and human umbilical vein endothelial cells (HUVEC) were cultured in the presence of levamisole and examined by solid-phase enzyme immunoassay to determine the level of expression of MHC class I, intercellular adhesion molecule 1 (ICAM-1), vascular cell-adhesion molecule-1 (VCAM-1), leukocyte integrin VLA-4, and lymphocyte-functional antigen (LFA-1) molecules. Adhesion of HL-60 and THP-1 myeloid cells to tumor cells was also evaluated. Tumor necrosis factor (TNF) at 10 ng/mL was used as a positive control for increasing adhesion molecule expression and cell-cell adhesion. The statistical significance of differences in cell surface molecule expression and functional adhesion between treated and control cells were tested by use of analysis of variance and the two-tailed Dunnett's test. Results: Treatment with levamisole (0.1 and 1 |ig/mL) caused the levels of MHC class I expression to increase approximately threefold above control levels on HCT-15 and LoVo colon tumor cells (P |
| Databáze: | OpenAIRE |
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