Anti-inflammatory Benzene Diamine Compound Inhibited Toll-Like Receptor 4-Mediated Inducible Nitric Oxide Synthase Expression and Nuclear Factor-KappaB Activation
| Autor: | Hyun-Mo Shin, Byung Hak Kim, Kyung Rak Min, Sang-Hum Jung, Young Gahp Yoon, Youngsoo Kim |
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| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharide
Pharmaceutical Science Diamines Gene Expression Regulation Enzymologic Cell Line Nitric oxide Mice chemistry.chemical_compound Animals Enzyme Inhibitors Receptor Pharmacology Toll-like receptor Innate immune system Dose-Response Relationship Drug biology Anti-Inflammatory Agents Non-Steroidal Benzene General Medicine Molecular biology Toll-Like Receptor 4 Fibronectin Nitric oxide synthase Biochemistry chemistry biology.protein TLR4 lipids (amino acids peptides and proteins) |
| Zdroj: | Biological and Pharmaceutical Bulletin. 28:908-911 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.28.908 |
| Popis: | Toll-like receptor 4 (TLR4) is known to play an important role in innate immune responses. In the present study, chemically synthetic compound of N(1)-benzyl-4-methylbenzene-1,2-diamine (BMD) was discovered to inhibit nitric oxide (NO) production in macrophages RAW 264.7 stimulated with lipopolysaccharide (LPS) or fibronectin as TLR4 activators. The BMD compound attenuated LPS-induced synthesis of both mRNA and protein of NO synthase (iNOS), and inhibited LPS or fibronectin-mediated iNOS promoter activity, indicating that the compound down-regulated iNOS expression at transcription level. As a mode of the anti-inflammatory action shown by BMD compound, inhibitory effect on nuclear factor (NF)-kappaB activation was also investigated in macrophages RAW 264.7 stimulated with the TLR4 activators. |
| Databáze: | OpenAIRE |
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