Primary structure and developmental expression of Bufo arenarum cellular nucleic acid-binding protein: Changes in subcellular localization during early embryogenesis

Autor: Nora B. Calcaterra, Pablo Armas, Marcelo O. Cabada
Jazyk: angličtina
Rok vydání: 2001
Předmět:
Cytoplasm
animal structures
DNA
Complementary
Time Factors
Databases
Factual
Blotting
Western
Biology
Immunolocalization
Ciencias Biológicas
purl.org/becyt/ford/1 [https]
Oogenesis
Escherichia coli
Animals
Tissue Distribution
Amino Acid Sequence
RNA
Messenger
Cloning
Molecular
purl.org/becyt/ford/1.6 [https]
Zinc finger
Cell Nucleus
Protein primary structure
Gene Expression Regulation
Developmental
RNA-Binding Proteins
Embryo
Cell Biology
Sequence Analysis
DNA
Bioquímica y Biología Molecular
Subcellular localization
Blastula
Blotting
Northern
Cchc Box
Early Embryogenesis
Molecular biology
Immunohistochemistry
Bufonidae
Gastrulation
DNA-Binding Proteins
embryonic structures
Nucleic acid
Oocytes
Bufo Arenarum
Cellular Nucleic Acid-Binding Protein
CIENCIAS NATURALES Y EXACTAS
Developmental Biology
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
DOI: 10.1046/j.1440-169x.2001.00551.x
Popis: A Bufo arenarum cellular nucleic acid-binding protein (bCNBP) full-length cDNA was cloned. bCNBP is a 19.4 kDa protein containing seven CCHC zinc finger motifs, an RGG box and a Ser-rich region. Amino acid comparisons showed high values of homology in vertebrates and smaller values in insects or inferior eukaryotes. Northern blot analysis during oogenesis and early development revealed two transcripts with different expressions of pattern behavior. One of them is present in all stages analyzed, whereas the other is only detected from the beginning of zygotic transcription. Immunocytochemistry assays carried out on sections of ovary and early embryos showed that there was no specific staining of previtellogenic oocytes. In early vitellogenic oocytes, in oocytes at stages V/VI and in embryos at early blastula stage, reaction was observed inside the cytoplasm. At mid-blastula stage, CNBP was mainly detected in the epiblast. At the late gastrula stage, two layers of cells were stained in the archenteron roof, in which the internal one presented as strong staining. Nuclei in this layer were stained even stronger than the cytoplasm. Changes in mRNA expression patterns, accompanied by changes in subcellular localization, suggest that CNBP might interact with both nuclear and cytoplasmic nucleic acids. Fil: Armas, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Cabada, Marcelo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina Fil: Calcaterra, Nora Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Databáze: OpenAIRE
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