In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(l-lysine) nanoparticles
| Autor: | Fei Tong, Haiying Jiang, YuJuan Liu, Fang Shan |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Lysine Pharmaceutical Science Bone Morphogenetic Protein 2 02 engineering and technology 01 natural sciences Micelle Polyethylene Glycols Rats Sprague-Dawley chemistry.chemical_compound Drug Delivery Systems International Journal of Nanomedicine BMP-2 Drug Discovery Polylysine Micelles Original Research chemistry.chemical_classification Drug Carriers General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Amino acid Biochemistry Liver Reperfusion Injury embryonic structures 0210 nano-technology Materials science Static Electricity Biophysics Bioengineering 010402 general chemistry Bone morphogenetic protein 2 Biomaterials In vivo PEG ratio Animals HI/RI GA-PEG-b-PLL Organic Chemistry 0104 chemical sciences Drug Liberation chemistry PIC micelles Glycyrrhetinic Acid Nanoparticles Nanocarriers Ethylene glycol |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Fang Shan,1 YuJuan Liu,1 Haiying Jiang,2 Fei Tong2 1Department of Physiology, Hexi University Medical College, Zhangye, 2Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, People’s Republic of China Abstract: Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly(L-lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with 1H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles. BMP-2 could be rapidly and efficiently encapsulated through the GA-PEG-b-PLL nanocarrier under physiological conditions, exhibiting efficient encapsulation and sustained release. In addition, the GA-PEG-b-PLL-mediated BMP-2 delivery system could target the liver against hepatic diseases as it has GA-binding receptors. The anti-hepatic ischemia/reperfusion injury (anti-HI/RI) effect of BMP-2/GA-PEG-b-PLL PIC micelles was investigated in rats using free BMP-2 and BMP-2/PEG-b-PLL PIC micelles as controls, and the results showed that BMP-2/GA-PEG-b-PLL PIC micelles indicated significantly enhanced anti-HI/RI property compared to BMP-2 and BMP-2/PEG-b-PLL. All results suggested that GA-PEG-b-PLL could be used as a potential BMP-2 nanocarrier. Keywords: GA-PEG-b-PLL, PIC micelles, BMP-2, HI/RI |
| Databáze: | OpenAIRE |
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