Identification and partial characterization of a Salp15 homolog from Ixodes ricinus
| Autor: | U. Seitzer, Doreen Beyer, J. S. Ahmed, J. Liu, M.A. Bakheit, S. Renneker, B. Kullmann |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Ixodes ricinus
Molecular Sequence Data Gene Expression Biology Microbiology Peripheral blood mononuclear cell law.invention law Complementary DNA parasitic diseases Animals Humans Amino Acid Sequence Salivary Proteins and Peptides Gene Cells Cultured Phylogeny chemistry.chemical_classification Base Sequence Ixodes Ricinus Sequence Analysis DNA biology.organism_classification Molecular biology Recombinant Proteins Amino acid Interleukin-10 Infectious Diseases Biochemistry chemistry Ixodes scapularis Insect Science Recombinant DNA Leukocytes Mononuclear Parasitology Female Sequence Alignment |
| Zdroj: | Ticks and tick-borne diseases. 5(3) |
| ISSN: | 1877-9603 |
| Popis: | The immunomodulatory molecule Salp15 is originally described in Ixodes scapularis and has been shown to inhibit CD4 T cell activation. Many Salp15 homologs have been described from Ixodes species, and all were well conserved at C-terminal residues that seem to be essential for the function of the protein. In this study, a gene sequence was amplified from cDNA isolated from engorged female I. ricinus ticks, which was predicted to generate a protein of 12.3 kDa. The protein displayed distinct amino acid differences from previously described I. ricinus Salp15 homologs, with amino acid identity ranging between 46.6% and 93.9%. It was referred to as I. ricinus Salp15-like protein. The protein showed 48.1% sequence identity to I. scapularis Salp15. We analyzed the effect of the recombinant I. ricinus Salp15-like protein on the production of cytokines from human peripheral blood mononuclear cells stimulated with LPS. The recombinant protein exerted no effect on the production of TNF-α and IL-6, but the production of IL-10 was dose-dependently reduced. It can be concluded that I. ricinus Salp15-like protein exerts an immunomodulatory effect on the host. The inhibition of IL-10 production may possibly lead to a retardation of B cell activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect