Effect of Sustained-Release PDGF and TGF-β on Cyclophosphamide-Induced Impaired Wound Healing
| Autor: | Anthony Spangenberger, Lawrence J. Bonassar, Azra Ashraf, Robert Valentini, Jeffrey Weinzweig, Victor Zaporojan, Kyoung Kim, Peter H. U. Lee |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Cyclophosphamide medicine.medical_treatment Pharmacology Rats Sprague-Dawley Transforming Growth Factor beta TGF beta signaling pathway medicine Animals Platelet-Derived Growth Factor Wound Healing biology business.industry Growth factor Rats Impaired wound healing Delayed-Action Preparations Anesthesia Drug delivery biology.protein Surgery Wound healing business Platelet-derived growth factor receptor medicine.drug Transforming growth factor |
| Zdroj: | Plastic and Reconstructive Surgery. 124:1118-1124 |
| ISSN: | 0032-1052 |
| DOI: | 10.1097/prs.0b013e3181b5a349 |
| Popis: | Background Proper wound healing is pivotal to successful surgical outcomes. Previous studies have shown that growth factors can be used to enhance tissue repair under impaired healing conditions. However, because of limited delivery methods, the growth factors in these studies were delivered either topically or as a single local administration. Methods Sixty Sprague-Dawley rats were divided equally into five groups and served as untreated normal controls or were implanted subcutaneously with a novel sustained-release drug delivery system through a dorsal incisional wound. This system delivered either transforming growth factor (TGF)-beta alone, platelet-derived growth factor (PDGF) alone, or TGF-beta and PDGF in combination, or served as unloaded sham controls. Wound healing was impaired in all treated rats by the administration of cyclophosphamide on days 1, 3, and 5. Wound tensile breaking strength was determined on days 4, 7, and 14. Results Sustained release of either TGF-beta or PDGF alone not only failed to improve the healing of cyclophosphamide-induced impaired wound healing but resulted in a paradoxical decrease in wound tensile breaking strength by day 7. However, the combined delivery of both TGF-beta and PDGF improved wound healing and significantly increased wound tensile breaking strength by day 7. Conclusions Sustained-release delivery of TGF-beta and PDGF in combination, but not separately, by a subcutaneously implanted drug delivery system significantly improves cyclophosphamide-induced impaired wound healing in rats. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|