Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India
| Autor: | R. M. Pillai, H. K. Mukhopadhyay, Mangadevi Nookala, M Srinivas, P. X. Antony, Amsaveni Sivaprakasam, Brindhalakshmi Balasubramanian, J. Thanislass |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Parvovirus Canine Sequence analysis animal diseases viruses India medicine.disease_cause Parvoviridae Infections Feces 03 medical and health sciences Dogs medicine Animals Dog Diseases Clade Gene Phylogeny Genetics Mutation General Immunology and Microbiology biology Phylogenetic tree Parvovirus Strain (biology) Canine parvovirus Sequence Analysis DNA General Medicine biology.organism_classification Virology 030104 developmental biology Capsid Proteins |
| Zdroj: | Acta Microbiologica et Immunologica Hungarica. 63:411-426 |
| ISSN: | 1588-2640 1217-8950 |
| DOI: | 10.1556/030.63.2016.010 |
| Popis: | The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyzed indicating emergence of newer variants of CPV in India. The phylogenetic analysis revealed that all the field CPV types were grouped in different subclades within two main clades, but away from the commercial vaccine strains. CPV-2b and CPV-2c types with unique mutations were found to be establishing in India apart from the prevailing CPV-2a type. Mutations and the positive selection of the mutants were found to be the major mechanism of emergence and evolution of parvovirus. Therefore, the incorporation of local strain in the vaccine formulation may be considered for effective control of CPV infections in India. |
| Databáze: | OpenAIRE |
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