Jaspine B induces nonapoptotic cell death in gastric cancer cells independently of its inhibition of ceramide synthase
| Autor: | Fabio Simbari, José Luis Abad, Anthony H. Futerman, Francesca Cingolani, Gemma Fabriàs, Mireia Casasampere, Josefina Casas |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Fabriàs, Gemma [0000-0001-7162-3772], Fabriàs, Gemma |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death Ceramide autophagy Cell Survival Acylation Apoptosis Vacuole QD415-436 Anticancer drugs Biochemistry Sphingolipid Wortmannin 03 medical and health sciences chemistry.chemical_compound Endocrinology methuosis Sphingosine Stomach Neoplasms Cell Line Tumor Autophagy Humans anticancer drug Liquid chromatography-mass spectrometry Ceramide synthase Research Articles liquid chromatography-mass spectrometry Methuosis Cell Death Cell Biology Cell biology 030104 developmental biology chemistry Vacuoles Cancer cell Pinocytosis sphingolipid Oxidoreductases |
| Zdroj: | Journal of Lipid Research, Vol 58, Iss 8, Pp 1500-1513 (2017) Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0022-2275 |
| Popis: | Sphingolipids (SLs) have been extensively investigated in biomedical research due to their role as bioactive molecules in cells. Here, we describe the effect of a SL analog, jaspine B (JB), a cyclic anhydrophytosphingosine found in marine sponges, on the gastric cancer cell line, HGC-27. JB induced alterations in the sphingolipidome, mainly the accumulation of dihydrosphingosine, sphingosine, and their phosphorylated forms due to inhibition of ceramide synthases. Moreover, JB provoked atypical cell death in HGC-27 cells, characterized by the formation of cytoplasmic vacuoles in a time and dose-dependent manner. Vacuoles appeared to originate from macropinocytosis and triggered cytoplasmic disruption. The pan-caspase inhibitor, z-VAD, did not alter either cytotoxicity or vacuole formation, suggesting that JB activates a caspase-independent cell death mechanism. The autophagy inhibitor, wortmannin, did not decrease JB-stimulated LC3-II accumulation. In addition, cell vacuolation induced by JB was characterized by single-membrane vacuoles, which are different from double-membrane autophagosomes. These findings suggest that JB-induced cell vacuolation is not related to autophagy and it is also independent of its action on SL metabolism. Copyright © 2017 by the American Society for Biochemistry and Molecular Biology, Inc. This work was supported by the Ministerio de Ciencia e Innovaci?n (Project CTQ2014-54743-R) and a predoctoral contract from Generalitat de Catalunya to F.C. A.H.F. is the Joseph Meyerhoff Professor of Biochemistry at the Weizmann Institute of Science. The authors thank Prof. R. Ghidoni and Dr. V. Gagliostro (University of Milan) for preliminary LC3-II data and Prof. A. Delgado for helpful discussions. The authors also thank E. Dalmau for excellent technical assistance. |
| Databáze: | OpenAIRE |
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