Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level
| Autor: | Carlos Pérez-Monter, Alejandro Álvarez-Arce, Natalia Nuño-Lambarri, Ivonne Escalona-Nández, Eva Juárez-Hernández, Norberto C. Chávez-Tapia, Misael Uribe, Varenka J. Barbero-Becerra |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
intestinal microbiota
DNA Bacterial Male QH301-705.5 Diet High-Fat DNA Ribosomal Catalysis Article Inorganic Chemistry Mice Non-alcoholic Fatty Liver Disease RNA Ribosomal 16S Animals Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Phylogeny inulin hepatic steatosis prebiotics A. muciniphila Organic Chemistry High-Throughput Nucleotide Sequencing General Medicine Akkermansia Sequence Analysis DNA Lipid Metabolism Computer Science Applications Gastrointestinal Microbiome Chemistry |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 23; Issue 2; Pages: 991 International Journal of Molecular Sciences, Vol 23, Iss 991, p 991 (2022) |
| ISSN: | 1422-0067 |
| Popis: | Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances. The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content. Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model. Mice exposed to a standard rodent diet or a fat-enriched diet, were supplemented or not, with inulin. Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing. Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group. We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia muciniphila increased by 5-fold with inulin supplementation. The family Prevotellaceae was also significantly increased in the fat-diet group. Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis. |
| Databáze: | OpenAIRE |
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