Gas chromatographic mass spectrometric determination of myo-inositol in humans utilizing a deuterated internal standard

Autor: Jan R. Andersen, Helge Harbo, J. E. Just Christensen, Elfinn Larsen, Boye Bertelsen, Gunnar Gregersen
Rok vydání: 1982
Předmět:
Zdroj: Biological Mass Spectrometry. 9:135-140
ISSN: 1096-9888
0306-042X
Popis: The isotopic dilution technique was used for determining the content of myo-inositol in human urine, plasma and haemolysed erythrocyte samples. A deuterated myo-inositol, synthesized from inosose-2 by base-catalysed exchange of hydrogens by deuterium, followed by reduction of the inosose with 2H2, was added as internal standard to the samples at an early stage in the analytical procedure. After separation and derivatization to the hexa-acetate, the gas chromatographic mass spectrometric analysis was carried out. A 25 m fused silica capillary column coated with methyl silicone was used, and the ions selected for monitoring were m/z 210 and m/z 214, which are characteristic and abundant fragment ions from unlabelled and hexadeuterated myo-inositolhexa-acetate, respectively. Calibration curves from water, urine, plasma and haemolysed erythrocytes show parallel, linear responses in the ratio between analyte and internal standard in the area of interest (0.2-2.0).
Databáze: OpenAIRE
Externí odkaz: