Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients
| Autor: | Beatriz Muñoz-Cobo, Rafael Borrás, Carlos Solano, David Navarro, Dayana Bravo, María Ángeles Clari, José Roberto Pineda, Mar Tormo, Gerardo Aguilar, Mónica Argueso, Elisa Costa, J. Blanquer |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) medicine.medical_specialty Neutropenia Fever medicine.drug_class Critical Illness Antibiotics Bacteremia Bloodstream infection Communicable Diseases Polymerase Chain Reaction Sensitivity and Specificity Blood culture law.invention Cohort Studies Critically ill patients law medicine Humans Diagnostic accuracy of the SeptiFast assay DNA Fungal Intensive care medicine Fungemia Aged Retrospective Studies Aged 80 and over medicine.diagnostic_test business.industry Critically ill General Medicine Middle Aged medicine.disease Intensive care unit Intensive Care Units Infectious Diseases Molecular Diagnostic Techniques Spain Etiology Female Reagent Kits Diagnostic business Real-time PCR |
| Zdroj: | International Journal of Infectious Diseases. 15:e326-e331 |
| ISSN: | 1201-9712 |
| DOI: | 10.1016/j.ijid.2011.01.003 |
| Popis: | Summary Objectives The objectives of this study were to compare the performance of the LightCycler SeptiFast Test MGRADE and conventional blood culture in the etiological diagnosis of febrile episodes occurring in neutropenic and critically ill patients (in the intensive care unit; ICU), and to assess the potential clinical value of the SeptiFast test in patient management. Methods A total of 86 febrile episodes occurring in 33 neutropenic patients and 53 ICU patients were analyzed. Blood samples for blood culture and SeptiFast testing were obtained at the onset of fever, before the implementation of empirical antimicrobial therapy. Results The overall microorganism-to-isolate agreement between the SeptiFast test and blood culture was 69% (κ=0.37) in neutropenic patients and 75% (κ=0.56) in ICU patients. The sensitivity of the SeptiFast assay for clinically relevant episodes of bacteremia and fungemia was 62% in neutropenic patients and 70% in ICU patients. Based on SeptiFast results, empirical treatments were deemed adequate in all but one of the febrile episodes. Nevertheless, early antibiotic treatment readjustment was judged feasible in most of clinically significant episodes overall. Conclusions The SeptiFast assay is a valuable ancillary method for the diagnosis of bloodstream infections in neutropenic and ICU patients. In these clinical settings, results of the SeptiFast assay may lead to a more targeted antibiotic therapy early after the onset of fever. |
| Databáze: | OpenAIRE |
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