Quantitative Evaluation of Equine Articular Cartilage Using Cationic Contrast-Enhanced Computed Tomography
| Autor: | Rachel C. Stewart, Jonathan D. Freedman, Brad B. Nelson, Brian D. Snyder, Amit Patwa, Chris E. Kawcak, Mark W. Grinstaff, Laurie R. Goodrich |
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| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
Cartilage Articular Basic Research Papers media_common.quotation_subject Biomedical Engineering Contrast Media Physical Therapy Sports Therapy and Rehabilitation Computed tomography Articular cartilage Osteoarthritis 03 medical and health sciences 0302 clinical medicine medicine Animals Immunology and Allergy Contrast (vision) Horses Range of Motion Articular Glycosaminoglycans media_common 030203 arthritis & rheumatology medicine.diagnostic_test Chemistry Cationic polymerization 030229 sport sciences medicine.disease Biomechanical Phenomena Disease Models Animal Contrast medium Tomography X-Ray Computed Biomedical engineering |
| Zdroj: | Cartilage |
| ISSN: | 1947-6043 1947-6035 |
| Popis: | Objective To investigate the diffusion trajectory of a cationic contrast medium (CA4+) into equine articular cartilage, and to assess normal and degenerative equine articular cartilage using cationic contrast-enhanced computed tomography (CECT). Design In the first experiment (Exp1), equine osteochondral specimens were serially imaged with cationic CECT to establish the diffusion time constant and time to reach equilibrium in healthy articular cartilage. In a separate experiment (Exp2), articular cartilage defects were created on the femoral trochlea (defect joint) in a juvenile horse, while the opposite joint was a sham-operated control. After 7 weeks, osteochondral biopsies were collected throughout the articular surfaces of both joints. Biopsies were analyzed for cationic CECT attenuation, glycosaminoglycan (GAG) content, mechanical stiffness (Eeq), and histology. Imaging, biochemical and mechanical data were compared between defect and control joints. Results Exp1: The mean diffusion time constant was longer for medial condyle cartilage (3.05 ± 0.1 hours) than lateral condyle cartilage (1.54 ± 0.3 hours, P = 0.04). Exp2: Cationic CECT attenuation was lower in the defect joint than the control joint ( P = 0.005) and also varied by anatomic location ( P = 0.045). Mean cationic CECT attenuation from the lateral trochlear ridge was lower in the defect joint than in the control joint (2223 ± 329 HU and 2667 ± 540 HU, respectively; P = 0.02). Cationic CECT attenuation was strongly correlated with both GAG (ρ = 0.79, P < 0.0001) and Eeq (ρ = 0.61, P < 0.0001). Conclusions The equilibration time of CA4+ into equine articular cartilage is affected by tissue volume. Quantitative cationic CECT imaging reflects the biochemical, biomechanical and histological state of normal and degenerative equine articular cartilage. |
| Databáze: | OpenAIRE |
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