Complex Toxin Profile of French Mediterranean Ostreopsis cf. ovata Strains, Seafood Accumulation and Ovatoxins Prepurification

Autor: Christine Herrenknecht, Jocelyn Harcouet, Zouher Amzil, Nicolas Chomérat, Philipp Hess, Rodolphe Lemée, Francesco Pisapia, Charline Brissard, Véronique Séchet, Fabienne Hervé
Přispěvatelé: Phytoxins laboratory, Nantes, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Mer, molécules et santé EA 2160 (MMS), Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN)-Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN), Laboratoire d'océanographie de Villefranche (LOV), Observatoire océanologique de Villefranche-sur-mer (OOVM), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Environnement Ressource de Bretagne Occidentale (LER-BO), Laboratoire Phycotoxines (PHYC), Dynamiques des Écosystèmes Côtiers (DYNECO), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Environnement Ressource de Bretagne Occidentale (LERBO), LITTORAL (LITTORAL)
Rok vydání: 2014
Předmět:
0106 biological sciences
Mediterranean climate
seafood contamination
Pharmaceutical Science
medicine.disease_cause
01 natural sciences
palytoxin & ovatoxins
chemistry.chemical_compound
Ostreopsis cf ovata
Palytoxin
Drug Discovery
Food science
lcsh:QH301-705.5
Pharmacology
Toxicology and Pharmaceutics (miscellaneous)
0303 health sciences
Ostreopsis cf. ovata
Anthozoa
Hemolysis
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Dinoflagellida
France
palytoxin &
In Vitro Techniques
Biology
[SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology
Article
ovatoxins
Microbiology
03 medical and health sciences
Cnidarian Venoms
culture
LC-MS/MS
hemolysis assay
Mediterranean Sea
medicine
Animals
Seawater
14. Life underwater
Shellfish
030304 developmental biology
Acrylamides
Sheep
Toxin
010604 marine biology & hydrobiology
medicine.disease
lcsh:Biology (General)
Seafood
chemistry
Sephadex
Marine Toxins
Marine toxin
Zdroj: Marine drugs
Marine drugs, MDPI, 2014, 12 (5), pp.2851-2876. ⟨10.3390/md12052851⟩
Marine drugs, 2014, 12 (5), pp.2851-2876. ⟨10.3390/md12052851⟩
Marine Drugs; Volume 12; Issue 5; Pages: 2851-2876
Marine Drugs
Marine Drugs, Vol 12, Iss 5, Pp 2851-2876 (2014)
Marine Drugs (1660-3397) (Mdpi Ag), 2014-05, Vol. 12, N. 5, P. 2851-2876
ISSN: 1660-3397
Popis: International audience; Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis OPEN ACCESS Mar. Drugs 2014, 12 2852 assay. Results obtained with these two methods were comparable, suggesting ovatoxins have hemolytic properties. An average of 223 μg· kg −1 of palytoxin equivalent of whole flesh was found, thus exceeding the threshold of 30 μg· kg −1 in shellfish recommended by the European Food Safety Authority (EFSA). Ostreopsis cells showed the same toxin profile both in situ and in laboratory culture, with ovatoxin-a (OVTX-a) being the most abundant analogue (~50%), followed by OVTX-b (~15%), p-PLTX (12%), OVTX-d (8%), OVTX-c (5%) and OVTX-e (4%). Ostreopsis cf. ovata produced up to 2 g of biomass per L of culture, with a maximum concentration of 300 pg PLTX equivalent cell −1. Thus, an approximate amount of 10 mg of PLTX-group toxins may be produced with 10 L of this strain. Toxin extracts obtained from collected biomass were purified using different techniques such as liquid-liquid partition or size exclusion. Among these methods, open-column chromatography with Sephadex LH20 phase yielded the best results with a cleanup efficiency of 93% and recovery of about 85%, representing an increase of toxin percentage by 13 fold. Hence, this purification step should be incorporated into future isolation exercises.
Databáze: OpenAIRE
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