The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites
| Autor: | Wojciech Tomaszewicz, Miroslaw Sobczak, Anna Mikuła, Michał Dziurka, Piotr Zdańkowski, Jan J. Rybczyński, Małgorzata Grzyb, Andrzej Kaźmierczak |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Somatic Embryogenesis Techniques Cytokinins Somatic embryogenesis Somatic cell QH301-705.5 polyamines carbohydrates Biology 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Auxin Biology (General) chemistry.chemical_classification amino acids Callose food and beverages Embryo General Medicine somatic embryogenesis phytohormones Multicellular organism 030104 developmental biology chemistry Biochemistry cytomorphology Ferns Gibberellin phenolic acids 010606 plant biology & botany Explant culture |
| Zdroj: | Cells, Vol 10, Iss 1388, p 1388 (2021) Cells Volume 10 Issue 6 |
| ISSN: | 2073-4409 |
| Popis: | Somatic embryogenesis is the formation of a plant embryo from a cell other than the product of gametic fusion. The need to recognize the determinants of somatic cell fate has prompted investigations on how endogenous factors of donor tissues can determine the pattern of somatic embryo origin. The undertaking of this study was enabled by the newly developed experimental system of somatic embryogenesis of the tree fern Cyathea delgadii Sternb., in which the embryos are produced in hormone-free medium. The contents of 89 endogenous compounds (such as sugars, auxins, cytokinins, gibberellins, stress-related hormones, phenolic acids, polyamines, and amino acids) and cytomorphological features were compared between two types of explants giving rise to somatic embryos of unicellular or multicellular origin. We found that a large content of maltose, 1-kestose, abscisic acid, biologically active gibberellins, and phenolic acids was characteristic for single-cell somatic embryo formation pattern. In contrast, high levels of starch, callose, kinetin riboside, arginine, and ethylene promoted their multicellular origin. Networks for visualization of the relations between studied compounds were constructed based on the data obtained from analyses of a Pearson correlation coefficient heatmap. Our findings present for the first time detailed features of donor tissue that can play an important role in the somatic-to-embryogenic transition and the somatic embryo origin. |
| Databáze: | OpenAIRE |
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