Heparin inhibits bovine testicular hyaluronidase activity in myocardium of dogs with coronary artery occlusion
| Autor: | Patricia E. Garrett, Robert A. Kloner, John Tumas, Michael L. Feldstein, Lee-Yuh Chaung, Robert Wolf, James E. Muller, Dietmar H. Glogar, Eugene Braunwald, George Ertl |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Necrosis Ischemia Hyaluronoglucosaminidase Coronary Disease Loading dose chemistry.chemical_compound Dogs In vivo Internal medicine Hyaluronic acid medicine Animals Myocardial infarction Enzyme Inhibitors Heparin business.industry Myocardium Heart medicine.disease chemistry Coronary occlusion Cardiology Female medicine.symptom Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | The American Journal of Cardiology. 53:941-944 |
| ISSN: | 0002-9149 |
| DOI: | 10.1016/0002-9149(84)90529-0 |
| Popis: | Bovine testicular hyaluronidase (BTH) reduces experimental myocardial infarct size and ameliorates electrocardiographic signs of ischemia. This study was done to determine if heparin, an in vitro inhibitor of hyaluronidase activity, blocks the action of BTH in the myocardium of dogs after coronary artery occlusion. BTH was administered intravenously as 5,000 NF units/kg at 0.5 and 2.5 hours after coronary occlusion. Heparin was administered intravenously as a 150-unit/kg loading dose, followed by 10 units/kg per hour i.v., beginning 15 minutes before coronary occlusion. The area of myocardial ischemia at risk was assessed by a radiolabeled microsphere technique; the area that developed necrosis was assessed by a histochemical technique. In vivo activity of BTH was assessed by a colorimetric analysis of the BTH substrate, i.e., hyaluronic acid (HA), extracted from myocardial tissue. For biochemical analysis of HA, the heart was divided into anterior myocardium, which included ischemic tissue and posterior nonischemic myocardium. The myocardial HA content of dogs treated with BTH plus heparin (anterior, 3.44 +/- 0.40 micrograms HA/mg protein; posterior, 3.69 +/- 0.33 micrograms HA/mg protein) was not significantly different from control (anterior, 3.61 +/- 0.29 micrograms HA/mg protein; posterior, 3.55 +/- 0.23 micrograms HA/mg protein). In contrast, BTH lowered myocardial HA content (anterior, 2.16 +/- 0.21 micrograms HA/mg protein; posterior, 2.08 +/- 0.14 micrograms HA/mg protein) compared with either BTH plus heparin or control groups in both anterior myocardium (p = 0.006) and posterior myocardium (p = 0.001).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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