Characterisation of an enterotoxin-negative, cytotoxin-positive strain of Clostridium sordellii

Autor:	V. Schué, Raymonde Girardot, Henri Monteil, G. A. Green
Rok vydání:	1996
Předmět:	Microbiology (medical) DNA Bacterial Molecular Sequence Data Clostridium sordellii Enzyme-Linked Immunosorbent Assay Enterotoxin medicine.disease_cause Microbiology Polymerase Chain Reaction law.invention Enterotoxins Open Reading Frames law Ileum Sequence Homology Nucleic Acid medicine Animals Humans Clostridiaceae Amino Acid Sequence Cloning Molecular Gene Polymerase chain reaction DNA Primers Clostridium biology Strain (chemistry) Base Sequence Toxin Clostridioides difficile Cytotoxins Nucleic acid sequence General Medicine biology.organism_classification Chromatography Ion Exchange Molecular biology Rabbits
Zdroj:	Journal of medical microbiology. 44(1)
ISSN:	0022-2615
Popis:	In ileal loop assay, ELISA and anion-exchange column chromatography, Clostridium sordellii strain 6018 was shown to produce a cytotoxin, but no detectable enterotoxin. DNA sequence and polymerase chain reaction analyses indicated that the lack of enterotoxin activity is not due to a lack of gene transcription, but to lack of a major portion of the enterotoxin gene. This is the first characterisation of such a strain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2466b7df03496151a924637e9c41419e https://pubmed.ncbi.nlm.nih.gov/8544213 Zobrazit plný text záznamu