Progestin binding sites in the rat hypothalamus pituitary and uterus
| Autor: | Martine Moguilewsky, Jean-Pierre Raynaud |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Cytoplasm
medicine.medical_specialty Norpregnadienes medicine.drug_class Clinical Biochemistry Hypothalamus Uterus Biology Biochemistry Endocrinology Estradiol Congeners Internal medicine medicine Animals Castration Binding site Receptor Molecular Biology Cell Nucleus Cerebral Cortex Pharmacology Progesterone Congeners Organic Chemistry In vitro Rats Dissociation constant medicine.anatomical_structure Pituitary Gland Female Receptors Progesterone Progestin hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Steroids. 30:99-109 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/0039-128x(77)90140-4 |
| Popis: | Specific, estrogen-inducible, nuclear radioactivity uptake has been demonstrated in the uterus, pituitary and hypothalamus of immature female rats after injection of a highly potent labelled progestin, R 5020 (17, 21-dimethyl-19-nor-pregna-4, 9-diene-3, 20-dione). A cytoplasmic progestin "receptor" has been characterized in these target tissues by an in vitro Dextran-coated charcoal adsorption method. R 5020 binds with the same intrinsic dissociation constant to the receptor present in these tissues; it dissociates at the same rate from the uterine and pituitary receptor (k-1 = 1 multiplied by 10(-2) min (-1), but about 6 times slower than progesterone. This evidence, together with the similar hormone specificity in the uterus and pituitary, suggests that the progestin "receptor" is a similar entity in central and peripheral target tissues. |
| Databáze: | OpenAIRE |
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