Use of enzyme-linked immunosorbent assays with chimeric fusion proteins to titrate antibodies against Epstein-Barr virus nuclear antigen 1
| Autor: | N Miyasaka, M Ohbayashi, Naoki Inoue, K Ezawa, S Harada, Y Nakamura, Fumihiko Ban, Kazuo Yanagi, H Nakajima, J Kuranari |
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| Rok vydání: | 1992 |
| Předmět: |
Microbiology (medical)
Herpesvirus 4 Human Recombinant Fusion Proteins viruses Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Antibodies Viral medicine.disease_cause Virus Immunoglobulin G Antigen hemic and lymphatic diseases medicine Humans Antigens Viral biology Antibody titer Fusion protein Epstein–Barr virus Virology Molecular biology Epstein-Barr Virus Nuclear Antigens biology.protein Antibody Epstein–Barr virus nuclear antigen 1 Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.30.6.1442-1448.1992 |
| Popis: | Two new enzyme-linked immunosorbent assays (ELISAs) with chimeric fusion polypeptides for the detection of human antibodies specific to Epstein-Barr virus nuclear antigen 1 (EBNA-1) are described. One is an indirect ELISA with affinity-purified beta-galactosidase-EBNA-1 fusion protein as the antigen. The other is a "sandwich" assay based on the use of anti-beta-galactosidase antibody to capture beta-galactosidase-EBNA-1 fusion proteins in bacterial extracts. A good correlation was shown between antibody titers determined by the ELISA with the EBNA-1 fusion proteins and those determined by a conventional anticomplement immunofluorescence test which is being widely performed with Raji cells for the purpose of research and clinical diagnosis. The advantage of the ELISAs for seroepidemiologic studies on Epstein-Barr virus was demonstrated by sensitive detection of marginal immunoglobulin G antibody to the EBNA-1 domain in serum samples from patients with infectious mononucleosis. |
| Databáze: | OpenAIRE |
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