Gene expression profiles in circulating tumor cells to predict prognosis in metastatic breast cancer patients
| Autor: | John W.M. Martens, Anieta M. Sieuwerts, Jaco Kraan, J Bolt-de Vries, P. van der Spoel, Agnes Jager, Bianca Mostert, Marcel Smid, A van Galen, Dieter Peeters, Caroline M. Seynaeve, Stefan Sleijfer, J.A. Foekens, Jan-Willem Gratama, F. E. de Jongh, Jacqueline M. Stouthard, Diederik F. S. Kehrer, Maxime P. Look, Luc Dirix, P. Hamberg |
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| Přispěvatelé: | Medical Oncology |
| Rok vydání: | 2014 |
| Předmět: |
Oncology
CA15-3 Adult medicine.medical_specialty CA 15-3 Breast Neoplasms Cohort Studies Circulating tumor cell Breast cancer SDG 3 - Good Health and Well-being Belgium Internal medicine Biomarkers Tumor Medicine Humans Prospective Studies Prospective cohort study Netherlands business.industry Gene Expression Profiling Hazard ratio Hematology Middle Aged medicine.disease Neoplastic Cells Circulating Prognosis Metastatic breast cancer Confidence interval Female Human medicine business |
| Zdroj: | Annals of oncology Annals of Oncology, 26(3), 510-516. Elsevier Ltd. |
| ISSN: | 1569-8041 0923-7534 |
| Popis: | Background: A circulating tumor cell (CTC) count is an established prognostic factor in metastatic breast cancer (MBC). Besides enumeration, CTC characterization promises to improve outcome prediction and treatment guidance. Having shown the feasibility of quantifying clinically relevant mRNA transcripts in CTCs, we determined the prognostic value of CTC gene expression in MBC. Patients and methods: CTCs were isolated and enumerated from blood of 197 MBC patients who were about to start first-line systemic therapy. Of these, 180 were assessable for quantification of mRNA expression by RT-qPCR in relation to time-to-treatment failure (TTF). A prognostic CTC gene profile was generated by leave-one-out cross validation in a 103 patient discovery set and validated in 77 patients. Additionally, all 180 patients were randomly divided into two equal sets to discover and validate a second prognostic profile. Results: CTC count predicted for TTF at baseline {>= 5 versus |
| Databáze: | OpenAIRE |
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