Heart failure increases protein expression and enzymatic activity of heme oxygenase-1 in the lung
| Autor: | Anthony J. Croatt, Chen-Fuh Lam, Zvonimir S. Katusic, Darcy M. Richardson, Karl A. Nath |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Oxygenase Time Factors Physiology Blotting Western Biology Critical Care and Intensive Care Medicine p38 Mitogen-Activated Protein Kinases Protein expression Rats Sprague-Dawley chemistry.chemical_compound Physiology (medical) Internal medicine Parenchyma medicine Animals Cyclic GMP Lung Heme Heart Failure chemistry.chemical_classification Nitrates business.industry Myocardium Proteins medicine.disease Enzyme assay Rats Heme oxygenase Enzyme medicine.anatomical_structure Endocrinology chemistry Biochemistry Heart failure Heme Oxygenase (Decyclizing) Models Animal biology.protein Hemoglobin business Cardiology and Cardiovascular Medicine Heme Oxygenase-1 |
| Zdroj: | Cardiovascular Research. 65:203-210 |
| ISSN: | 0008-6363 |
| DOI: | 10.1016/j.cardiores.2004.09.001 |
| Popis: | Objective : Heart failure (HF) cell or siderophages are pulmonary macrophages that phagonicytize erythrocytes leaked from the congested capillaries due to HF. Degradation of erythrocytes and hemoglobin increases concentrations of heme in the lung. We hypothesized that the HF-induced increase in the concentration of heme up-regulates the expression and enzymatic activity of heme oxygenase (HO)-1 in the lung. Methods : Using the aortocaval (AC) fistula model of HF, we examined the following parameters 8–10 weeks after the creation of the fistula: morphological changes in the lung by Prussian blue iron and immunohistochemical staining, HO-1 protein expression and activity in the rat lungs, and concentrations of nitrite/nitrate (NO x −) and cyclic guanosine 3′,5′-monophospate (cGMP) of the lung homogenates. Results : Iron-stained siderophages were observed only in the lungs of rats with AC fistula. Protein level and enzyme activity of HO-1 were significantly enhanced in the lung of HF rats. NO x − concentrations of the two groups were similar, but cGMP was elevated in the lung of AC fistula rats (0.34 ± 0.06 vs. 0.89 ± 0.20 pmol/mg protein, P =0.025). Staining of serial sections of the lung tissues demonstrated induction of HO-1 co-localized to iron-stained siderophages. Conclusions : HF causes increased pulmonary HO-1 expression and activity, which emanates largely from siderophages. Up-regulation of HO-1 may have pulmonary protective in HF. |
| Databáze: | OpenAIRE |
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