Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging

Autor: Guy Schoehn, Clarisse Uwizeye, Gilles Curien, Rachel Templin, Samuel C. Zeeman, Yannick Schwab, Davide Dal Bo, Denis Falconet, Fabien Chevalier, Serena Flori, Christine Moriscot, Florence Courtois, Claire Seydoux, Jean-Baptiste Keck, Benoit Gallet, Johan Decelle, Nicole L. Schieber, Pierre-Henri Jouneau, Giovanni Finazzi, Guillaume Allorent
Přispěvatelé: LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Photosymbiose, Modélisation et Exploration des Matériaux (MEM), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Light Photosynthesis & Metabolism (Photosynthesis), Institut de biologie structurale (IBS - UMR 5075), Calcul des Variations, Géométrie, Image (CVGI), Laboratoire Jean Kuntzmann (LJK), Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, European Molecular Biology Laboratory [Grenoble] (EMBL), Department of Biology [ETH Zürich] (D-BIOL), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Défi X-Life grant from CNRS, CEA DRFimpulsion FIB-Bioprogram, Auvergne-Rhône-Alpes Region, Fondation Recherche Medicale (FRM), FEDER and the GIS-Infrastructures en Biologie Santé et Agronomie (IBiSA), ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), ANR-17-CE05-0029,MoMix,Modélisation de la Mixotrophie chez l'algue extrêmophile Galdieria sulphuraria(2017), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), ANR-15-IDEX-0002,UGA,IDEX UGA(2015), European Project: 833184, ChloroMito, European Project: 654248,H2020,H2020-INFRADEV-1-2014-1,CORBEL(2015)
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
Cell biology
Light
Bioenergetics
MESH: Mitochondria
Acclimatization
Science
General Physics and Astronomy
MESH: Acclimatization
MESH: Imaging
Three-Dimensional
Cellular imaging
Photosynthesis
01 natural sciences
Article
General Biochemistry
Genetics and Molecular Biology
Pyrenoid
MESH: Microalgae
03 medical and health sciences
Imaging
Three-Dimensional
Phytoplankton
Microalgae
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Plastids
Plastid
Cellular microbiology
Trophic level
Biomass (ecology)
Multidisciplinary
biology
Ecology
MESH: Energy Metabolism
fungi
MESH: Plastids
General Chemistry
biology.organism_classification
MESH: Light
Mitochondria
030104 developmental biology
Diatom
MESH: Subcellular Fractions
Energy Metabolism
Subcellular Fractions
MESH: Phytoplankton
010606 plant biology & botany
Zdroj: Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), pp.1049. ⟨10.1038/s41467-021-21314-0⟩
Nature Communications, 2021, 12 (1), pp.1049. ⟨10.1038/s41467-021-21314-0⟩
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Nature Communications, 12 (1)
ISSN: 2041-1723
DOI: 10.1038/s41467-021-21314-0
Popis: Eukaryotic phytoplankton have a small global biomass but play major roles in primary production and climate. Despite improved understanding of phytoplankton diversity and evolution, we largely ignore the cellular bases of their environmental plasticity. By comparative 3D morphometric analysis across seven distant phytoplankton taxa, we observe constant volume occupancy by the main organelles and preserved volumetric ratios between plastids and mitochondria. We hypothesise that phytoplankton subcellular topology is modulated by energy-management constraints. Consistent with this, shifting the diatom Phaeodactylum from low to high light enhances photosynthesis and respiration, increases cell-volume occupancy by mitochondria and the plastid CO2-fixing pyrenoid, and boosts plastid-mitochondria contacts. Changes in organelle architectures and interactions also accompany Nannochloropsis acclimation to different trophic lifestyles, along with respiratory and photosynthetic responses. By revealing evolutionarily-conserved topologies of energy-managing organelles, and their role in phytoplankton acclimation, this work deciphers phytoplankton responses at subcellular scales.
Nature Communications, 12 (1)
ISSN:2041-1723
Databáze: OpenAIRE
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