Generation of High Dose Inhalable Effervescent Dispersions against Pseudomonas aeruginosa Biofilms

Autor: Hareir Al-Kassimy, Rachith Kalgudi, Chahinez Houacine, Muhammad Yaqoob, Amina Ferraz, Somto Madueke, Jakub Zurek, Aram Mohammed, Nicholas Hawkins, Mohammed Gulrez Zariwala, Hisham Al-Obaidi
Rok vydání: 2020
Předmět:
H100
Diffusing-wave spectroscopy
Silver
Co-amorphous
Pharmaceutical Science
Salt (chemistry)
Microbial Sensitivity Tests
02 engineering and technology
effervescent
03 medical and health sciences
chemistry.chemical_compound
dry powder inhaler
Ciprofloxacin
Administration
Inhalation
Pharmacology (medical)
Fourier transform infrared spectroscopy
Thermal analysis
Tartrates
030304 developmental biology
Pharmacology
chemistry.chemical_classification
0303 health sciences
Sodium bicarbonate
Chemistry
Organic Chemistry
Biofilm
Dry Powder Inhalers
H811
Silicon Dioxide
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
Sodium Bicarbonate
Biofilms
Pseudomonas aeruginosa
Pyocyanine
Tartaric acid
Molecular Medicine
Particle
Glycolipids
0210 nano-technology
Research Paper
Biotechnology
Nuclear chemistry
Zdroj: Pharmaceutical Research
ISSN: 1573-904X
0724-8741
DOI: 10.1007/s11095-020-02878-w
Popis: Abstract Purpose Novel particle engineering approach was used in this study to generate high dose inhalable effervescent particles with synergistic effects against Pseudomonas aeruginosa biofilms. Methods Spray dried co-amorphous salt of ciprofloxacin (CFX) and tartaric acid (TA) was prepared and coated with external layer of sodium bicarbonate and silica coated silver nanobeads. Design of experiments (DOE) was used to optimize physicochemical properties of particles for enhanced lung deposition. Results Generated particles were co-amorphous CFX/TA showing that CFX lost its zwitterionic form and exhibiting distinct properties to CFX/HCl as assessed by FTIR and thermal analysis. Particles exhibited mass mean aerodynamic diameter (MMAD) of 3.3 μm, emitted dose of 78% and fine particle dose of 85%. Particles were further evaluated via antimicrobial assessment of minimum inhibitory concentrations (MIC) and minimum biofilm eradication concentration (MBEC). MIC and MBEC results showed that the hybrid particles were around 3–5 times more effective when compared to CFX signifying that synergistic effect was achieved. Diffusing wave spectroscopy results showed that the silver containing particles had a disruptive effect on rheological properties as opposed to silver free particles. Conclusions Overall, these results showed the potential to use particle engineering to generate particles that are highly disruptive of bacterial biofilms.
Databáze: OpenAIRE
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