Duffy Blood Group Genotyping in Thai Blood Donors
| Autor: | Oytip Nathalang, Kanokpol Siriphanthong, Siriporn Nathalang, Pawinee Kupatawintu, Kamphon Intharanut |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Genotyping medicine.medical_specialty Blood transfusion Genotype medicine.medical_treatment Clinical Biochemistry Blood Donors Receptors Cell Surface PCR-SSP Polymerase Chain Reaction Duffy blood group Isoantibodies Young Adult Asian People Gene Frequency medicine Humans Allele Allele frequency Alleles Blood type Base Sequence Transfusion Medicine business.industry Biochemistry (medical) Allele frequencies Transfusion medicine DNA Sequence Analysis DNA General Medicine Middle Aged Thailand Thais Phenotype Immunology Female Original Article Duffy Blood-Group System business |
| Zdroj: | Annals of Laboratory Medicine |
| ISSN: | 2234-3814 2234-3806 |
| Popis: | Background Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequence-specific primers (PCR-SSP), and the probability of obtaining compatible blood for alloimmunized patients was assessed. Methods Five hundred blood samples from Thai blood donors of the National Blood Centre, Thai Red Cross Society, were included. Only 200 samples were tested with anti-Fya and anti-Fyb using the gel technique. All 500 samples and four samples from a Guinea family with the Fy(a-b-) phenotype were genotyped by using PCR-SSP. Additionally, the probability of obtaining antigen-negative red blood cells (RBCs) for alloimmunized patients was calculated according to the estimated FY allele frequencies. Results The FY phenotyping and genotyping results were in 100% concordance. The allele frequencies of FY*A and FY*B in 500 central Thais were 0.962 (962/1,000) and 0.038 (38/1,000), respectively. Although the Fy(a-b-) phenotype was not observed in this study, FY*BES/FY*BES was identified by PCR-SSP in the Guinea family and was confirmed by DNA sequencing. Conclusions Our results confirm the high frequency of the FY*A allele in the Thai population, similar to that of Asian populations. At least 500 Thai blood donors are needed to obtain two units of antigen-negative RBCs for the Fy(a-b+) phenotype. |
| Databáze: | OpenAIRE |
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