Non-genomic effects of progesterone on the signaling function of G protein-coupled receptors
| Autor: | Falk Fahrenholz, Katja Burger, Gerald Gimpl |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Receptors
Neuropeptide medicine.medical_specialty Receptors Vasopressin Time Factors Biophysics Stimulation CHO Cells Cycloheximide Biology Non-genomic effect Calcium signal Biochemistry Cell Line chemistry.chemical_compound Structure-Activity Relationship Species Specificity Structural Biology Internal medicine Cricetinae Progesterone receptor Genetics medicine Tumor Cells Cultured Animals Humans G protein-coupled receptor Receptor Molecular Biology Progesterone Calcium signaling Protein Synthesis Inhibitors Dose-Response Relationship Drug Cell Biology Ligand (biochemistry) Oxytocin receptor Kinetics Endocrinology chemistry Receptors Oxytocin Anisotropy Calcium Receptors Cholecystokinin Signal Transduction |
| Zdroj: | FEBS Letters. (1-2):25-29 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(99)01668-3 |
| Popis: | Progesterone at concentrations between 10 microM and 200 microM affected the calcium signaling evoked by ligand stimulation of G protein-coupled receptors expressed in several cell lines. At 160 microM progesterone the signaling of all receptors was completely abolished. The effect of progesterone was fast, reversible and was not prevented by cycloheximide indicating its non-genomic nature. Overall, the action of progesterone was more cell type-specific than receptor-specific. Our results are in contrast to a recent report [Grazzini, E., Guillon, G., Mouillac, B. and Zingg, H.H. (1998) Nature 392, 509-512] in which a direct high-affinity interaction between the oxytocin receptor and progesterone was suggested. |
| Databáze: | OpenAIRE |
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