Investigation of Charge-State-Dependent Compaction of Protein Ions with Native Ion Mobility–Mass Spectrometry and Theory

Autor:	Amber D. Rolland, Lejla S. Biberic, James S. Prell
Rok vydání:	2022
Předmět:	Ions Glutamate Dehydrogenase Protein Conformation Structural Biology Ion Mobility Spectrometry Concanavalin A Gases Lactoglobulins Molecular Dynamics Simulation Spectroscopy
Zdroj:	Journal of the American Society for Mass Spectrometry. 33:369-381
ISSN:	1879-1123 1044-0305
DOI:	10.1021/jasms.1c00351
Popis:	The precise relationship between native gas-phase protein ion structure, charge, desolvation, and activation remains elusive. Much evidence supports the Charge Residue Model for native protein ions formed by electrospray ionization, but scaling laws derived from it relate only to overall ion size. Closer examination of drift tube CCSs across individual native protein ion charge state distributions (CSDs) reveals deviations from global trends. To investigate whether this is due to structure variation across CSDs or contributions of long-range charge-dipole interactions, we performed
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24560553a136af2fcdd7e5a8450471e6 https://doi.org/10.1021/jasms.1c00351 Zobrazit plný text záznamu