Putative pectate lyase PLL12 and callose deposition through polar CALS7 are necessary for long-distance phloem transport in Arabidopsis

Autor: Lothar Kalmbach, Matthieu Bourdon, Ilya Belevich, Josip Safran, Adrien Lemaire, Jung-ok Heo, Sofia Otero, Bernhard Blob, Jérôme Pelloux, Eija Jokitalo, Ykä Helariutta
Přispěvatelé: Department of Plant Molecular Biology, Université de Lausanne = University of Lausanne (UNIL), Sainsbury Laboratory Cambridge University (SLCU), University of Cambridge [UK] (CAM), Biologie du Fruit, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Helsinki Institute of Life Science (HiLIFE), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), HiLIFE - Institute of Biotechnology [Helsinki] (BI), Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Apollo - University of Cambridge Repository
Rok vydání: 2023
Předmět:
Zdroj: Current Biology-CB
Current Biology-CB, 2023, ⟨10.1016/j.cub.2023.01.038⟩
ISSN: 0960-9822
1879-0445
Popis: In plants, the phloem distributes photosynthetic products for metabolism and storage over long distances. It relies on specialized cells, the sieve elements, which are enucleated and interconnected through large so-called sieve pores in their adjoining cell walls. Reverse genetics identified PECTATE LYASE LIKE 12 (PLL12) as critical for plant growth and development. Using genetic complementations, we established that PLL12 is required exclusively late during sieve element differentiation. Structural homology modeling, enzyme inactivation, and overexpression suggest a vital role for PLL12 in sieve element specific pectin remodeling. While short distance symplastic diffusion is unaffected, the pll12 mutant is unable to accommodate sustained plant development due to an incapacity to accommodate increasing hydraulic demands on phloem long distance transport as the plant grows – a defect that is aggravated when combined with another sieve element specific mutant callose synthase 7 (cals7). Establishing CALS7 as a specific sieve pore marker, we investigated the sub36 cellular dynamics of callose deposition in the developing sieve plate. Using fluorescent CALS7 then allowed identifying structural defects in pll12 sieve pores that are moderate at the cellular level but become physiologically relevant due to the serial arrangement of sieve elements in the sieve tube. Overall, pectin degradation through PLL12 appears subtle in quantitative terms. We therefore speculate that PLL12 may act as a regulator to locally remove homogalacturonan thus potentially enabling further extracellular enzymes to access and modify the cell wall during sieve pore maturation.
Databáze: OpenAIRE
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