Potential circulating miRNA signature for early detection of NSCLC
| Autor: | Sirous Zeinali, Kambiz Sheikhy, Ayda Arab, Arda Kiani, Elham Tafsiri, Shiva Irani, Morteza Karimipoor |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Oncology Circulating mirnas Cancer Research medicine.medical_specialty Lung Neoplasms Early detection Biology Bioinformatics Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Internal medicine microRNA Genetics medicine Humans Stage (cooking) Molecular Biology Early Detection of Cancer Demography Training set Receiver operating characteristic Gene Expression Profiling Reproducibility of Results Cancer Middle Aged medicine.disease Gene Expression Regulation Neoplastic MicroRNAs Circulating MicroRNA 030104 developmental biology ROC Curve Area Under Curve 030220 oncology & carcinogenesis Female |
| Zdroj: | Cancer Genetics. :150-158 |
| ISSN: | 2210-7762 |
| Popis: | Circulating microRNAs (c-miRNAs) are promising biomarkers for screening, early detection and prognosis of cancer. The purpose of this investigation was to identify a panel of c-miRNAs in plasma that could contribute to early detection of non-small cell lung cancer (NSCLC). We profiled the expression of 44 unique plasma miRNAs in training set of 34 NSCLC patients and 20 matched healthy individuals by miRCURY LNA™ Universal RT microRNA PCR Panel and calculated dysregulation fold changes using the 2-ΔΔCt equation. Selected plasma miRNAs were then validated by SYBR green q-RT PCR using an independent validation set of plasma samples from NSCLC patients (n: 72) and NC (n: 50). In the validation set, the receiver operating characteristic (ROC) curves were generated for four miRNAs. In the training set, 17 miRNAs were significantly up-regulated and nine were down-regulated in the plasma from NSCLC patients versus matched normal controls. Four miRNAs (miR-21, miR-328, miR-375 and miR-141) were selected for validating their diagnostic value in the testing set. ROC plot analysis showed that a high specificity (98%) and sensitivity (82.7%) in miR-141 in comparing early NSCLC patient and controls. So among these four plasma miRNAs only miR-141 could be promising biomarkers for early detection of NSCLC. In addition to, we found a significant positive correlation between stage and miR-21 expression level (95% CI: 0.687-0.863; p-value 0.0001). Considering the accessibility and stability of circulating miRNAs, plasma miR-141 is a useful biomarker early detection of NSCLC as a supplement in future screening studies. |
| Databáze: | OpenAIRE |
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