Topical application of zinc oxide nanoparticles reduces bacterial skin infection in mice and exhibits antibacterial activity by inducing oxidative stress response and cell membrane disintegration in macrophages
| Autor: | Ranjit Kumar Mehta, Soumitra Mohanty, Chandan Goswami, Baskarlingam Vaseeharan, Mitali Sengupta, Avinash Padhi, Avinash Sonawane, Rashmirekha Pati |
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| Rok vydání: | 2013 |
| Předmět: |
Staphylococcus aureus
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Skin infection medicine.disease_cause Bacterial cell structure Monocytes Microbiology Mycobacterium Mice medicine Animals Humans General Materials Science Skin Mice Inbred BALB C Mycobacterium Infections biology Mycobacterium smegmatis Macrophages Hemolysin Staphylococcal Infections Antimicrobial medicine.disease biology.organism_classification Anti-Bacterial Agents Oxidative Stress Biofilms Molecular Medicine Nanoparticles Female Staphylococcal Skin Infections Zinc Oxide Intracellular Oxidative stress |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 10(6) |
| ISSN: | 1549-9642 |
| Popis: | Here we studied immunological and antibacterial mechanisms of zinc oxide nanoparticles (ZnO-NPs) against human pathogens. ZnO-NPs showed more activity against Staphylococcus aureus and least against Mycobacterium bovis -BCG. However, BCG killing was significantly increased in synergy with antituberculous-drug rifampicin. Antibacterial mechanistic studies showed that ZnO-NPs disrupt bacterial cell membrane integrity, reduce cell surface hydrophobicity and down-regulate the transcription of oxidative stress-resistance genes in bacteria. ZnO-NP treatment also augmented the intracellular bacterial killing by inducing reactive oxygen species production and co-localization with Mycobacterium smegmatis -GFP in macrophages. Moreover, ZnO-NPs disrupted biofilm formation and inhibited hemolysis by hemolysin toxin producing S. aureus . Intradermal administration of ZnO-NPs significantly reduced the skin infection, bacterial load and inflammation in mice, and also improved infected skin architecture. We envision that this study offers novel insights into antimicrobial actions of ZnO-NPs and also demonstrates ZnO-NPs as a novel class of topical anti-infective agent for the treatment of skin infections. From the Clinical Editor This in-depth study demonstrates properties of ZnO nanoparticles in infection prevention and treatment in several skin infection models, dissecting the potential mechanisms of action of these nanoparticles and paving the way to human applications. |
| Databáze: | OpenAIRE |
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