Long-term persistence and bacterial transformation potential of transplastomic plant DNA in soil

Autor: Alessandra Pontiroli, Paolo Nannipieri, Maria Teresa Ceccherini, Timothy M. Vogel, Pascal Simonet, Walter Wildi, Elisabeth Kay, Jean-Michel Monier, John Poté
Přispěvatelé: Ampère, École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Dipartimento Sci Suolo & Nutr Pianta, Université de Florence, Forel Institute, University of Geneva [Switzerland], Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Dipartimento Scienza del Suolo e Nutrizione della Pianta, Università degli Studi di Firenze [Firenze], École Centrale de Lyon ( ECL ), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Ampère (AMPERE), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Chloroplasts
Genetically modified crops
Polymerase Chain Reaction
HORIZONTAL GENE-TRANSFER
law.invention
chemistry.chemical_compound
Soil
law
Transgenes
Polymerase chain reaction
Soil Microbiology
2. Zero hunger
0303 health sciences
Acinetobacter
Soil bacteria
IN-SITU
Genetic transfer
DNA
Chloroplast
General Medicine
Horizontal gene transfer
Plants
Genetically Modified
GENOME
ESCHERICHIA-COLI
RESISTANCE GENES
Soil microbiology
Plasmids
DNA
Bacterial
PSEUDOMONAS-FLUORESCENS
Gene Transfer
Horizontal
Transgenic plant DNA
Biology
Microbiology
Persistence
03 medical and health sciences
NATURAL TRANSFORMATION
Tobacco
MICROCOSMS
Genome
Chloroplast
Molecular Biology
Gene
030304 developmental biology
[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology
Bacteria
Base Sequence
030306 microbiology
STRAIN BD413
DNA
15. Life on land
Plant Leaves
Transformation (genetics)
chemistry
ACINETOBACTER-CALCOACETICUS
Transformation
Bacterial
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Transplastomic plant
Zdroj: Research in Microbiology
Research in Microbiology, Elsevier, 2010, 161 (5), pp.326-334. ⟨10.1016/j.resmic.2010.04.009⟩
Research in Microbiology, Elsevier, 2010, 161 (5), pp.326-334. 〈10.1016/j.resmic.2010.04.009〉
ISSN: 0923-2508
DOI: 10.1016/j.resmic.2010.04.009⟩
Popis: International audience; The long-term physical persistence and biological activity of transplastomic plant DNA (transgenes contained in the chloroplast genome) either purified and added to soil or naturally released by decaying tobacco leaves in soil was determined. Soil microcosms were amended with transplastomic tobacco leaves or purified plant DNA and incubated for up to 4 years. Total DNA was extracted from soil and the number of transgenes (aadA, which confers resistance to both spectinomycin and streptomycin) was quantified by quantitative PCR. The biological activity of these transgenes was assessed by transformation in the bacterial strain Acinetobacter sp. BD413(pBAB2) in vitro. While the proportion of transgenes recovered increased with the increasing amount of transplastomic DNA added, plant DNA was rapidly degraded over time. The number of transgenes recovered decreased about 10,000 fold within 2 weeks. Data reveal, however, that a small fraction of the plant DNA escaped degradation. Transgene sequences were still detected after 4 years and transformation assays showed that extracted DNA remained biologically active and could still transform competent cells of Acinetobacter sp. BD413(pBAB2). The approach presented here quantified the number of transgenes (based on quantitative PCR of 50% of the gene) released and persisting in the environment over time and provided new insights into the fate of transgenic plant DNA in soil. (C) 2010 Elsevier Masson SAS. All rights reserved.
Databáze: OpenAIRE
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