Novel liquid chromatographic method for simultaneous estimation of pioglitazone and glimepiride in rat plasma by solid phase extraction: application to preclinical pharmacokinetic studies
| Autor: | Prashant B Musmade, Nayanabhirama Udupa, Kranti B. Talole, Praful B. Deshpande, Shriram M. Pathak, Arumugam Karthik, Sureshwar Pandey |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Bioanalysis Drug Evaluation Preclinical Pharmacokinetics Drug Discovery medicine Animals Hypoglycemic Agents Drug Interactions Solid phase extraction Rats Wistar Chromatography High Pressure Liquid Chromatography Pioglitazone Chemistry Solid Phase Extraction Extraction (chemistry) Reproducibility of Results Half-life Reference Standards Rats Glimepiride Sulfonylurea Compounds Linear range Area Under Curve Calibration Spectrophotometry Ultraviolet Thiazolidinediones Half-Life medicine.drug |
| Zdroj: | Arzneimittelforschung. 61:23-31 |
| ISSN: | 1616-7066 0004-4172 |
| Popis: | The need for a reliable bioanalytical method is of primary importance during preclinical studies. The aim of the present study was simultaneous determination of pioglitazone (CAS 111025-46-8) (PIO) and glimepiride (CAS 93479-97-1) (GLM) in plasma of rats. A high-performance liquid chromatographic method has been developed and validated using C18 column and UV detector. A mobile phase composed of acetonitrile and ammonium acetate buffer pH 4.5 in the ratio of 55:45%. The plasma samples clean-up was carried out using solid phase cartridges. The method was in the linear range of 50-8000 ng/mL for PIO and 50-2000 ng/mL for GLM. The coefficient of regression was found to beor = 0.99. Precision and accuracy were within the acceptable limits, as indicated by relative standard deviation varying from 1.5 to 6.1% for PIO and 3.1 to 7.0% for GLM whereas the accuracy ranged from 97.0 to 106.4% for PIO and 96.5 to 106.4% for GLM. The mean extraction recovery was found to be 90.2 +/- 4.5, 76.8 +/- 2.8 and 85.2 +/- 5.2% for PIO, GLM and internal standard, respectively. Moreover, PIO and GLM were stable in plasma, up to 30 days of storage at -70 degrees C and after being subjected to bench top, auto-sampler, and three freeze-thaw cycles. The developed method was applied for preclinical pharmacokinetic studies. |
| Databáze: | OpenAIRE |
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