Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
| Autor: | Gizem Ertürk Bergdahl, Bo Mattiasson, Martin Hedström |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
medicine.medical_treatment Capacitive sensing Bioengineering Standard solution Electric Capacitance 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Article Degradation 010608 biotechnology medicine Molecular Biology chemistry.chemical_classification Enzymatic activity Protease Chromatography biology 010405 organic chemistry Chemistry Substrate (chemistry) Electrochemical Techniques General Medicine Enzyme assay 0104 chemical sciences Enzyme Immunoglobulin G biology.protein Degradation (geology) Capacitive sensor Digestion Peptide Hydrolases Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-019-03006-0 |
| Popis: | A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications. Electronic supplementary material The online version of this article (10.1007/s12010-019-03006-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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