mRNA BRAF expression helps to identify papillary thyroid carcinomas in thyroid nodules independently of the presence of BRAFV600E mutation
| Autor: | Ana Carolina Trindade Guilhen, Alfio José Tincani, Elaine Cristina Morari, Marjory Alana Marcello, Priscila Pereira Costa Araújo, Laura Sterian Ward |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Proto-Oncogene Proteins B-raf Thyroid nodules Pathology medicine.medical_specialty endocrine system diseases Biopsy Fine-Needle Gene Expression Malignancy Pathology and Forensic Medicine Papillary thyroid cancer Diagnosis Differential Thyroid carcinoma Predictive Value of Tests Biopsy Biomarkers Tumor medicine Carcinoma Humans Prospective Studies RNA Messenger Thyroid Neoplasms Thyroid Nodule Thyroid cancer medicine.diagnostic_test business.industry Cell Biology Middle Aged medicine.disease Carcinoma Papillary Thyroid Cancer Papillary Predictive value of tests Mutation Female business |
| Zdroj: | Pathology - Research and Practice. 208:489-492 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2012.05.013 |
| Popis: | Literature has consistently shown associations of BRAFV600E mutation with papillary thyroid cancer clinical features. However, the clinical utility of BRAF expression has not been clinically explored so far. We studied 67 thyroid nodules (32 benign nodules and 35 PTC cases). BRAF mRNA expression levels measured by a quantitative real-time PCR and a PCR-RFLP were used to identify BRAFV600E mutation. BRAF mRNA expression was significantly higher in malignant (198.2±373.9 AU) than in benign (4.1±6.9 AU) nodules (p |
| Databáze: | OpenAIRE |
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