Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo
| Autor: | Seyed Moein Moghimi, Donald S. Backos, Nirmal K. Banda, Guankui Wang, V. Michael Holers, Lin-Ping Wu, Dmitri Simberg, Barbara Brenneman, James I. Griffin, Fangfang Chen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Complement Pathway Alternative Biomedical Engineering Nanoparticle Bioengineering Nanotechnology Protein Corona 02 engineering and technology Article 03 medical and health sciences Immune system Coated Materials Biocompatible In vivo Humans General Materials Science Electrical and Electronic Engineering Magnetite Nanoparticles Chemistry Dextrans Complement C3 biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics Complement system 030104 developmental biology Biophysics 0210 nano-technology |
| Zdroj: | Nature Nanotechnology. 12:387-393 |
| ISSN: | 1748-3395 1748-3387 |
| DOI: | 10.1038/nnano.2016.269 |
| Popis: | When nanoparticles are intravenously injected into the body, complement proteins deposit on the surface of nanoparticles in a process called opsonization. These proteins prime the particle for removal by immune cells and may contribute toward infusion-related adverse effects such as allergic responses. The ways complement proteins assemble on nanoparticles have remained unclear. Here, we show that dextran-coated superparamagnetic iron oxide core-shell nanoworms incubated in human serum and plasma are rapidly opsonized with the third complement component (C3) via the alternative pathway. Serum and plasma proteins bound to the nanoworms are mostly intercalated into the nanoworm shell. We show that C3 covalently binds to these absorbed proteins rather than the dextran shell and the protein-bound C3 undergoes dynamic exchange in vitro. Surface-bound proteins accelerate the assembly of the complement components of the alternative pathway on the nanoworm surface. When nanoworms pre-coated with human plasma were injected into mice, C3 and other adsorbed proteins undergo rapid loss. Our results provide important insight into dynamics of protein adsorption and complement opsonization of nanomedicines. |
| Databáze: | OpenAIRE |
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