Pseudolaric acid B inhibits proliferation in SW579 human thyroid squamous cell carcinoma
| Autor: | Rui Li, Jinghua Yu, Peiyou Ren, Guang Chen, Bianbian Xue, Xiao fang Yu, Yalin Wang, Minghui Yan, Chunyu Liu, Ting Zhong, Chunyan Dai |
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| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Cell cycle checkpoint Cell Antineoplastic Agents Apoptosis Biology Biochemistry Cell Movement Tubulin Cell Line Tumor Autophagy Genetics medicine Humans Thyroid Neoplasms Molecular Biology SW579 thyroid squamous cell carcinoma cell line antitumor Cell Proliferation Cell growth pseudolaric acid B Cell migration Articles Cell cycle Cytostasis Cell biology G2 Phase Cell Cycle Checkpoints medicine.anatomical_structure Oncology Cell culture Carcinoma Squamous Cell Cancer research Molecular Medicine Diterpenes Drug Screening Assays Antitumor Apoptosis Regulatory Proteins |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2015.4418 |
| Popis: | Primary squamous cell carcinoma of the thyroid is a rare and aggressive type of neoplasm, which is routinely treated with surgery; however, despite this, survival time is not commonly more than six months. Thus, the aim of the present study was to determine the efficacy of pseudolaric acid (PAB) as a therapeutic agent. PAB is an antitubulin agent, and in the present study, inhibition of the SW579 thyroid squamous cell carcinoma cell line by PAB was investigated. PAB was found to inhibit SW579 cell growth in a time‑ and dose‑dependent manner via interference in α‑tubulin polymerization. However, the inhibitory role of PAB in SW579 cells was not predominantly due to apoptosis, but was due to the cytostatic status resulting from cell cycle arrest. The present study proposes that this is the underlying mechanism of the antitumor properties of PAB. During cytostatis, autophagy was activated to sustain cell survival and SW579 cell migration was inhibited. Nuclear p53 expression was observed to be reduced, however the role of reduced p53 requires further investigation. Therefore, PAB induced cytostasis, which inhibited SW579 cell growth and therefore may function as an antitubulin therapeutic agent. |
| Databáze: | OpenAIRE |
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