Effect of nisin on microbiome-brain-gut axis neurochemicals by Escherichia coli-induced diarrhea in mice
| Autor: | Chunjie Wang, Meiling He, Puchun Niu, Aorigele Chen, Fuxiang Bao, Zhifeng Jia, Jin Xu, Xin Zhang, Siming Gao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial Diarrhea Male Serotonin Duodenum Dopamine Ileum medicine.disease_cause Microbiology Jejunum 03 medical and health sciences chemistry.chemical_compound Cecum Mice Norepinephrine 0302 clinical medicine Intestinal mucosa medicine Escherichia coli Animals Intestinal Mucosa Nisin Aquaporin 3 Brain Biodiversity Small intestine Gastrointestinal Microbiome 030104 developmental biology Infectious Diseases medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis |
| Zdroj: | Microbial pathogenesis. 119 |
| ISSN: | 1096-1208 |
| Popis: | The effects of nisin on the neurochemicals, Aquaporin-3 (AQP-3) and intestinal microorganisms in the brain-gut axis of mice were analyzed by using enzyme linked immune sorbent assay (ELISA) and high throughput sequencing in this investigation, to further revealed the relationship between intestinal flora abundance in mice and neurochemicals in the brain-gut axis. Using HE staining found damage of structure of small intestine villi in the model group (Escherichia coli O1, E. coli O1). Compared with normal control and ciprofloxacin groups, using ELISA showed that nisin increased the highest norepinephrine (NE) expression in the brain, expression of 5-hydroxytryptamine (5-HT) and dopamine (DA) in the duodenum, and increased the expression of AQP-3 in jejunum. Using high-throughput sequencing showed the highest diversity of cecal microflora in nisin group (ACE-index = 1417.25, Chao1-index = 1378.45), but the cecal microflora in the negative control group (ACE-index = 969.54, Chao1-index = 340.29) exhibited the lowest species diversity. Our data indicated that nisin regulates neurochemicals, AQP-3 and cecal microflora imbalance in mice. |
| Databáze: | OpenAIRE |
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