In Silico and Transcriptional Analysis of Carbohydrate Uptake Systems of Streptomyces coelicolor A3(2)
| Autor: | Harald Nothaft, Maximilian Schlicht, Milton H. Saier, Fritz Titgemeyer, Kerstin Mahr, Ralph Bertram |
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| Rok vydání: | 2004 |
| Předmět: |
Genomics and Proteomics
Transcription Genetic Molecular Sequence Data Biology Microbiology Streptomyces Substrate Specificity chemistry.chemical_compound Ribose Phosphoenolpyruvate Sugar Phosphotransferase System Molecular Biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction Permease Membrane transport protein Streptomyces coelicolor Membrane Transport Proteins Biological Transport Gene Expression Regulation Bacterial PEP group translocation Chromosomes Bacterial biology.organism_classification Major facilitator superfamily chemistry Biochemistry Symporter biology.protein Carbohydrate Metabolism ATP-Binding Cassette Transporters |
| Zdroj: | Journal of Bacteriology. 186:1362-1373 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Streptomyces coelicolor is the prototype for the investigation of antibiotic-producing and differentiating actinomycetes. As soil bacteria, streptomycetes can metabolize a wide variety of carbon sources and are hence vested with various specific permeases. Their activity and regulation substantially determine the nutritional state of the cell and, therefore, influence morphogenesis and antibiotic production. We have surveyed the genome of S. coelicolor A3(2) to provide a thorough description of the carbohydrate uptake systems. Among 81 ATP-binding cassette (ABC) permeases that are present in the genome, we found 45 to encode a putative solute binding protein, an essential feature for carbohydrate permease function. Similarity analysis allowed the prediction of putative ABC systems for transport of cellobiose and cellotriose, α-glucosides, lactose, maltose, maltodextrins, ribose, sugar alcohols, xylose, and β-xylosides. A novel putative bifunctional protein composed of a substrate binding and a membrane-spanning moiety is likely to account for ribose or ribonucleoside uptake. Glucose may be incorporated by a proton-driven symporter of the major facilitator superfamily while a putative sodium-dependent permease of the solute-sodium symporter family may mediate uptake of galactose and a facilitator protein of the major intrinsic protein family may internalize glycerol. Of the predicted gene clusters, reverse transcriptase PCRs showed active gene expression in 8 of 11 systems. Together with the previously surveyed permeases of the phosphotransferase system that accounts for the uptake of fructose and N -acetylglucosamine, the genome of S. coelicolor encodes at least 53 potential carbohydrate uptake systems. |
| Databáze: | OpenAIRE |
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