Antimicrobial Susceptibility of Standard Strains of Nontuberculous Mycobacteria by Microplate Alamar Blue Assay
| Autor: | Li Wan, Li Li Zhao, Kanglin Wan, Haican Liu, Lu Lu Lian, Yi Jiang, Jingrui Zhang, Xiuqin Zhao, Guilian Li |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Bacterial Diseases
Drugs and Devices Infectious Disease Control lcsh:Medicine Mycobacterium chelonae Microbial Sensitivity Tests Mycobacterium abscessus Toxicology Microbiology Slowly growing Mycobacteria Species Specificity Mycobacterium bolletii Oxazines medicine lcsh:Science Biology Microbial Pathogens Multidisciplinary biology lcsh:R Isoniazid Bacteriology Nontuberculous Mycobacteria bacterial infections and mycoses biology.organism_classification Antimicrobial Virology Bacterial Pathogens Anti-Bacterial Agents Infectious Diseases Pharmacodynamics Xanthenes Medical Microbiology Medicine lcsh:Q Nontuberculous mycobacteria Mycobacterium simiae Research Article medicine.drug |
| Zdroj: | PLoS ONE, Vol 8, Iss 12, p e84065 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0084065 |
| Popis: | In this study, 24 standard nontuberculous mycobacteria (NTM) species strains including 12 slowly growing mycobacteria strains and 12 rapidly growing mycobacteria strains were subjected to drug susceptibility testing using microplate Alamar Blue assay-based 7H9 broth. The most active antimicrobial agents against the 24 NTM strains were streptomycin, amikacin, the fluoroquinolones, and the tetracyclines. Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium simiae are resistant to most antimicrobial agents. The susceptibility results of this study from 24 NTM standard strains can be referenced by clinicians before susceptibility testing for clinical isolates is performed or when conditions do not allow for susceptibility testing. The application of broth-based methods is recommended by the Clinical and Laboratory Standards Institute, and the documentation of the susceptibility patterns of standard strains of mycobacteria can improve the international standardization of susceptibility testing methods. |
| Databáze: | OpenAIRE |
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