Disruption of the myostatin gene in porcine primary fibroblasts and embryos using zinc-finger nucleases
| Autor: | Hong-xiao Zhang, Honglin Liu, Xian-Ju Huang, Kai Xiong, Ling Qin, Huili Wang |
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| Rok vydání: | 2013 |
| Předmět: |
Microinjections
Swine Mutant Food Genetically Modified Primary Cell Culture knockout Myostatin medicine Animals Blastocyst Fibroblast gene Molecular Biology Gene knockout Cells Cultured Zinc finger Deoxyribonucleases biology Electroporation Muscles fungi Zinc Fingers Cell Biology General Medicine Exons Fibroblasts musculoskeletal system porcine Zinc finger nuclease Molecular biology Meat Products medicine.anatomical_structure zinc-finger nucleases (ZFNs) Gene Knockdown Techniques biology.protein Research Article |
| Zdroj: | Molecules and Cells |
| ISSN: | 0219-1032 |
| Popis: | Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin loss-of-function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos. |
| Databáze: | OpenAIRE |
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