Heterogeneity of disease and clones of community-onset methicillin-resistant Staphylococcus aureus in children attending a paediatric hospital in Belgium

Autor: M. Doyen, Claire Nonhoff, Anne Vergison, Olivier Denis, Ariane Deplano, R. De Mendonça, J. Brauner, G. Mascart, A. Nobre Machado
Rok vydání: 2012
Předmět:
Male
Pediatrics
MRSA
Disease
medicine.disease_cause
Polymerase Chain Reaction
Cystic fibrosis
Cohort Studies
Belgium
Epidemiology
Genotype
Cluster Analysis
Prospective Studies
Child
Children
General Medicine
Staphylococcal Infections
Hospitals
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Community-Acquired Infections
Treatment Outcome
Infectious Diseases
Staphylococcus aureus
Child
Preschool
Female
Methicillin-Resistant Staphylococcus aureus
Microbiology (medical)
medicine.medical_specialty
HA-MRSA
Adolescent
Virulence Factors
CO-MRSA
paediatrics
Young Adult
Antibiotic resistance
medicine
Humans
business.industry
Infant
Newborn
Genetic Variation
Infant
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
medicine.disease
Methicillin-resistant Staphylococcus aureus
Multilocus sequence typing
business
Multilocus Sequence Typing
Zdroj: Clinical Microbiology and Infection. 18(8):769-777
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2011.03637.x
Popis: The increase in the number of methicillin-resistant Staphylococcus aureus (MRSA) infections in children has prompted paediatricians to broaden th empirical treatment of common community-onset (CO) infections in children in several countries. Most European countries have reported low rates of CO-MRSA infection, but limited data on paediatric CO-MRSA infections are available. A prospective study was conducted from January 2002 to December 2004 in Brussels. CO-MRSA was defined as MRSA first detected by culture within 48 h of admission or in outpatients. Clinical and epidemiological data were recorded. CO-MRSA strains were genotyped by pulsed-field gel electrophoresis and multilocus sequence typing. Staphylococcal chromosomal cassette mec, toxin (Panton–Valentin leukocidin (PVL), toxic shock syndrome toxin 1, and Eta/b), enterotoxin and antibiotic resistance genes were detected by PCR. The antibiotic resistance phenotype was determined by disk diffusion. S. aureus was isolated in 1681 children. Among these, 107 harboured MRSA. Fifty-one children were colonized or infected by CO-MRSA, 20% of whom had no healthcare exposure. Twelve infants
Databáze: OpenAIRE
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