Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin
| Autor: | Li Hou, S. J. Kohlhepp, D. N. Gilbert |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Membrane Fluidity
Swine Biology Phosphatidylinositols Models Biological Fluorescence Kidney Tubules Proximal Cell membrane Andrology medicine Membrane fluidity Tobramycin Animals Pharmacology (medical) Cells Cultured Phospholipids Antibacterial agent Pharmacology Kidney Chromatography Cell Membrane Aminoglycoside Photobleaching Infectious Diseases medicine.anatomical_structure Gentamicin Gentamicins Research Article medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 38:2169-2171 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.38.9.2169 |
| Popis: | The surface membrane properties of LLC-PK1 cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L- alpha-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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