REGULATORY MUTATIONS OF INOSITOL BIOSYNTHESIS IN YEAST: ISOLATION OF INOSITOL-EXCRETING MUTANTS
| Autor: | Barry Reiner, Susan A. Henry, Miriam L. Greenberg |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Mutant
Saccharomyces cerevisiae Genes Recessive Investigations Membrane Lipids chemistry.chemical_compound Genes Regulator Genetics Inositol Phospholipids Genes Dominant chemistry.chemical_classification biology Myo-Inositol-1-Phosphate Synthase ATP synthase Structural gene biology.organism_classification Molecular biology Enzyme chemistry Biochemistry Mutation biology.protein Inositol-3-phosphate synthase |
| Zdroj: | Genetics. 100:19-33 |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/100.1.19 |
| Popis: | The enzyme inositol-1-phosphate synthase (I-1-P synthase), product of the INO1 locus, catalyzes the synthesis of inositol-1-phosphate from the substrate glucose-6-phosphate. The activity of this enzyme is dramatically repressed in the presence of inositol. By selecting for mutants which overproduce and excrete inositol, we have identified mutants constitutive for inositol-1-phosphate synthase as well as a mutation in phospholipid biosynthesis. Genetic analysis of the mutants indicates that at least three loci (designated OPI1, OPI2 and OPI4) direct inositol-mediated repression of I-1-P synthase. Mutants of these loci synthesize I-1-P synthase constitutively. Three loci are unlinked to each other and to INO1, the structural gene for the enzyme. A mutant of a fourth locus, OPI3, does not synthesize I-1-P synthase constitutively, despite its inositol excretion phenotype. This mutant is preliminarily identified as having a defect in phospholipid synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|