Intensity interrogation near cutoff resonance for label-free cellular profiling
| Autor: | Yousef Nazirizadeh, Robert Horvath, Martina Gerken, Aurel Prosz, Volker Behrends, Norbert Orgovan, Christine Selhuber-Unkel, Ye Fang, Ann M. Ferrie |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Lipid Bilayers Biosensing Techniques 02 engineering and technology Bioinformatics Article Cell Line Receptors G-Protein-Coupled 03 medical and health sciences Microtiter plate Cell Line Tumor Animals Humans Cutoff Label free Multidisciplinary business.industry Bilayer Optical Imaging Resonance Fibroblasts 021001 nanoscience & nanotechnology Cutoff frequency High-Throughput Screening Assays Rats 030104 developmental biology Reduced size Optoelectronics 0210 nano-technology business Refractive index |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep24685 |
| Popis: | We report a method enabling intensity-based readout for label-free cellular assays and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems. |
| Databáze: | OpenAIRE |
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