Washless double staining of unfixed nuclei for flow cytometric analysis of DNA and a nuclear antigen (Ki-67 or bromodeoxyuridine)
| Autor: | Børge Thing Mortensen, Jette Christiansen, Ib Jarle Christensen, Jørgen K. Larsen |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Lymphocyte
Biophysics Biology Pathology and Forensic Medicine chemistry.chemical_compound Endocrinology Antigen medicine Humans Lymphocytes Cell Nucleus Staining and Labeling DNA synthesis Nuclear Proteins DNA DNA Neoplasm Cell Biology Hematology Flow Cytometry Molecular biology Staining Cell nucleus Ki-67 Antigen medicine.anatomical_structure Bromodeoxyuridine chemistry Leukemia Myeloid Antigen KI-67 |
| Zdroj: | Cytometry. 12:429-437 |
| ISSN: | 1097-0320 0196-4763 |
| DOI: | 10.1002/cyto.990120508 |
| Popis: | Washless methods for double staining of nuclear antigen and DNA in unfixed nuclei were compared with established methods for staining of fixed cells. The methods were tested on phytohemagglutinin (PHA)-stimulated normal human blood lymphocytes for the double staining of (1) Ki-67 antigen and DNA and (2) bromodeoxyuridine (BrdUrd) and DNA, in continuously BrdUrd-labeled cells. With respect to the discrimination between antigen-positive and -negative subpopulations, there was no statistically significant differences between the results from direct (Ki-67) or indirect, (Ki-67 or BrdUrd) washless staining of unfixed nuclei and the results from staining of fixed cells. Washless staining of unfixed nuclei was found to be rapid and simple and resulted in greater precision of the DNA analysis and in less aggregation and loss of cells. |
| Databáze: | OpenAIRE |
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