Two bHLH transcription factors, bHLH48 and bHLH60, associate with phytochrome interacting factor 7 to regulate hypocotyl elongation in Arabidopsis
| Autor: | Chuanwei Yang, Chang Liu, Sha Huang, Steve A. Kay, Jose L. Pruneda-Paz, Qinyi Ma, Yue Zeng, Lin Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
PIF7 QH301-705.5 Regulator Arabidopsis General Biochemistry Genetics and Molecular Biology Hypocotyl bHLH48/bHLH60 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gene expression Basic Helix-Loop-Helix Transcription Factors Biology (General) Transcription factor transcriptional activity Phytochrome biology Arabidopsis Proteins hypocotyl elongation DNA binding ability biology.organism_classification Cell biology 030104 developmental biology chemistry 030217 neurology & neurosurgery Function (biology) DNA |
| Zdroj: | Cell Reports, Vol 35, Iss 5, Pp 109054-(2021) |
| ISSN: | 2211-1247 |
| Popis: | Summary: The basic helix-loop-helix (bHLH) transcription factor PHYTOCHROME-INTERACTING FACTOR 7 (PIF7) is a central regulator that promotes stem growth by activating growth-related gene expression during shade-avoidance responses. Studying the co-factors of PIF7 can facilitate understanding of the mechanism of PIFs and light signal transduction. Here, we describe the identification of two bHLH transcription factors, bHLH48 and bHLH60 (bHLH48/bHLH60), as essential partners for PIF7-dependent modulation of hypocotyl elongation and function downstream of phytochrome B. These two bHLH factors display DNA binding activity and interact with PIF7. Genetic analysis indicated that bHLH48/bHLH60 and PIF7 are interdependent in promoting hypocotyl elongation. Chromatin immunoprecipitation sequencing (ChIP-seq) analysis identified the substantially overlapping downstream targets of bHLH60 and PIF7. Biochemical analysis revealed that bHLH48/bHLH60 enhance the DNA binding ability of PIF7. These results provide evidence that bHLH48/bHLH60 act as positive partners of PIF7 for mutual benefit in the regulation of hypocotyl elongation. |
| Databáze: | OpenAIRE |
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