TNPO3 Is Required for HIV-1 Replication after Nuclear Import but prior to Integration and Binds the HIV-1 Core
| Autor: | Patricio Perez, Felipe Diaz-Griffero, Maritza Lienlaf, Abraham L. Brass, Jose Carlos Valle-Casuso, Yang Yang, Francesca Di Nunzio, Nathalie J. Arhel, Natalia Reszka |
|---|---|
| Přispěvatelé: | Department of Microbiology and Immunology, Albert Einstein College of Medicine [New York], Virologie moléculaire et vaccinologie, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Ragon Institute of Massachusetts General Hospital, Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Cell Nucleus
Cytoplasm Virus Integration Immunology Human immunodeficiency virus (HIV) Active Transport Cell Nucleus HIV Infections MESH: Active Transport Cell Nucleus Biology medicine.disease_cause Virus Replication Microbiology gag Gene Products Human Immunodeficiency Virus MESH: HIV-1 03 medical and health sciences Virology medicine MESH: Protein Binding Humans 030304 developmental biology Cell Nucleus 0303 health sciences MESH: Humans 030306 microbiology MESH: Cytoplasm MESH: Virus Replication virus diseases MESH: HIV Infections beta Karyopherins MESH: gag Gene Products Human Immunodeficiency Virus Replication (computing) 3. Good health Virus-Cell Interactions Core (game theory) Insect Science [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology HIV-1 Nuclear transport MESH: beta Karyopherins MESH: Virus Integration Protein Binding |
| Zdroj: | Journal of Virology Journal of Virology, American Society for Microbiology, 2012, 86 (10), pp.5931-6. ⟨10.1128/JVI.00451-12⟩ Journal of Virology, 2012, 86 (10), pp.5931-6. ⟨10.1128/JVI.00451-12⟩ |
| ISSN: | 0022-538X 1098-5514 |
| DOI: | 10.1128/JVI.00451-12⟩ |
| Popis: | TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import. |
| Databáze: | OpenAIRE |
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