Locating a Blood Pressure Quantitative Trait Locus Within 117 kb on the Rat Genome
| Autor: | Bina Joe, Michael R. Garrett, John P. Rapp, Haijin Meng |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Untranslated region
Quantitative Trait Loci Congenic Gene Expression Blood Pressure Nerve Tissue Proteins Locus (genetics) Biology Quantitative trait locus Kidney Polymorphism Single Nucleotide Species Specificity Animals Congenic Internal Medicine Animals Coding region Gene Oligonucleotide Array Sequence Analysis Genetics Expressed sequence tag Genome Rats Inbred Dahl Gene Expression Profiling Chromosome Mapping Kidney metabolism Rats Inbred Strains Rats |
| Zdroj: | Hypertension. 45:451-459 |
| ISSN: | 1524-4563 0194-911X |
| DOI: | 10.1161/01.hyp.0000154678.64340.7f |
| Popis: | Previously, a blood pressure (BP) quantitative trait locus (QTL) on rat chromosome 9 (RNO9) was localized to a Resp18 ), which also mapped in the 2.4-cM BP QTL interval. Additional substitution mapping located the QTL to Resp18 . Nucleotide variants were identified between S and R genomic DNA of Resp18 in the coding, 5′ regulatory and 3′ untranslated regions. The coding sequence variation (T/C) occurs in exon 2 and predicts an amino acid change (Ile/Val) in the protein product. Resp18 was considered a differentially expressed positional candidate for the QTL. To fine-map the BP QTL, we constructed a congenic strain with a smaller introgressed region. Compared with the S rat, this strain (1) had significantly lower BP, (2) did not contain the R form of Resp18 , and (3) did not retain the rather spectacular differential expression of Resp18. Together, these results demonstrate that a BP QTL independent of Resp18 exists within the newly defined 117-kb QTL region on RNO9. |
| Databáze: | OpenAIRE |
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